Benzodiazepines in Urine on Raptor Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Q1	Q3 Quantifier	Q3 Qualifier
1.	7-Aminoclonazepam	0.86	286.0	222.2	250.3
2.	7-Aminoflunitrazepam	1.15	284.2	135.2	240.2
3.	Flurazepam	1.29	388.1	315.1	288.2
4.	Midazolam	1.41	326.1	291.2	244.2
5.	N-Desmethylflunitrazepam	1.76	300.0	254.1	225.2
6.	Lorazepam	1.79	321.0	275.1	302.9
7.	Oxazepam	1.86	287.1	241.0	104.1
8.	Clonazepam	1.88	315.9	270.2	214.1
9.	Nitrazepam	1.88	282.1	236.1	207.2
10.	Desalkylflurazepam	1.93	289.0	140.1	165.1
11.	2-Hydroxyethylflurazepam	2.00	333.1	246.1	166.0
12.	Nordiazepam	2.07	271.0	140.0	208.0
13.	Clobazam	2.13	301.1	259.1	224.0
14.	Estazolam	2.18	294.9	267.1	241.0
15.	Triazolam	2.19	343.1	308.2	315.0
16.	Flunitrazepam	2.19	314.4	239.1	211.0
17.	Temazepam	2.23	301.2	255.0	177.1
18.	Alprazolam	2.27	309.4	281.1	205.0
19.	Diazepam-d5 (IS)	2.45	290.0	154.1	-
20.	Diazepam	2.47	284.8	193.1	222.2

LC_CF0581

Column

Raptor Biphenyl (cat.# 9309A5E)

Dimensions:

50 mm x 3.0 mm ID

Particle Size:

2.7 µm

Guard Column:

Raptor Biphenyl EXP 5 mm, 3.0 mm ID, 2.7 µm (cat.# 9309A0253)

Temp.:

40 °C

Sample

Benzodiazepine custom mix

Conc.:

A 50 ng/mL standard was prepared in urine. 50 µL of 50 ng/mL urine standard was diluted with 950 µL of water prior to analysis (20x dilution).

Inj. Vol.:

20 µL

Mobile Phase

2 mM Ammonium formate and 0.2% formic acid in water

0.2% Formic acid in methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.8	50	50
2.50	0.8	5	95
3.00	0.8	5	95
3.01	0.8	50	50
4.50	0.8	50	50

Detector

ABSCIEX API 4000 MS/MS

Ion Source:

TurboIonSpray^®

Ion Mode:

ESI+

Mode:

Scheduled MRM

Instrument

API LC-MS/MS

Download PDF

Printing tips! To print chromatograms full page, change the print settings in the print dialogue window of your PDF reader software: 1) "fit" or "fit to page", 2) "landscape" for wide chromatograms or "portrait" for tall chromatograms.

More Search Options

Benzodiazepines in Urine on Raptor Biphenyl by LC-MS/MS

Restek Domestic Customer Service

Thank you

Message not sent

	Raptor EXP Guard Column Cartridges cat.# 9309A0253 Qty:
	Raptor Biphenyl LC Columns cat.# 9309A5E Qty: