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THC + Metabolites in Urine on Raptor Biphenyl by LC-MS/MS

THC + Metabolites in Urine on Raptor Biphenyl by LC-MS/MS

PeakstR (min)Q1Q3 QuantifierQ3 Qualifier
1.THC-OH-d3 (IS)1.94334.35196.26-
2.THC-OH1.94331.32193.18105.11
3.THC-COOH-d3 (IS)2.09348.35196.24-
4.THC-COOH2.09345.36193.18119.16
5.Δ9-THC-d3 (IS)2.32318.42196.25-
6.Δ9-THC2.33315.32193.18135.16
image
LC_CF0583
ColumnRaptor Biphenyl (cat.# 9309A5E)
Dimensions:50 mm x 3.0 mm ID
Particle Size:2.7 µm
Guard Column:Raptor Biphenyl EXP 5 mm, 3.0 mm ID, 2.7 µm (cat.# 9309A0253)
Temp.:40 °C
SampleCustom
Conc.: A 50 ng/mL standard was prepared in urine. 1 mL of the 50 ng/mL urine standard was diluted with 2 mL of 0.1% formic acid in 50:50 water:methanol prior to analysis.
Inj. Vol.:2 µL
Mobile Phase
A:0.1% Formic acid in water
B:0.1% Formic acid in methanol
Time (min)Flow (mL/min)%A%B
0.000.7004060
2.50.7000100
3.00.7000100
3.010.7004060
4.00.7004060
DetectorWaters Xevo TQ-S
Ion Source:Waters Zspray™ ESI
Ion Mode:ESI+
InstrumentWaters
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cannabinoids

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