Pure Chromatography
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Fentanyl, Metabolite, and Analogues in Urine on Raptor Biphenyl by LC-MS/MS

LC_CF0591
Column | Raptor Biphenyl (cat.# 9309552) | ||||||||||||||||||||
Dimensions: | 50 mm x 2.1 mm ID | ||||||||||||||||||||
Particle Size: | 5 µm | ||||||||||||||||||||
Temp.: | 30 °C | ||||||||||||||||||||
Sample | |||||||||||||||||||||
Conc.: | A 10 ng/mL standard was prepared in urine. The internal standard was fortified to a final concentration of 1 ng/mL. An 80 μL aliquot of the 10 ng/mL urine standard was diluted with 320 μL of 70:30 water:methanol solution (5-fold dilution) prior to analysis. | ||||||||||||||||||||
Inj. Vol.: | 5 µL | ||||||||||||||||||||
Mobile Phase | |||||||||||||||||||||
A: | 0.1% Formic acid in water | ||||||||||||||||||||
B: | 0.1% Formic acid in methanol | ||||||||||||||||||||
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Detector | MS/MS | ||||||||||||||||||||
Ion Mode: | ESI+ | ||||||||||||||||||||
Instrument | HPLC |
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