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Steroids in Serum on Raptor Biphenyl by LC-MS/MS
Conc. listed is the final concentration following sample preparation.
|Column||Raptor Biphenyl (cat.# 9309A12)|
|Dimensions:||100 mm x 2.1 mm ID|
|Particle Size:||2.7 µm|
|Guard Column:||Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)|
|Conc.:||500 µL of mobile phase B was added to 250 µL of fortified beagle serum (100-400 ng/mL). The sample was vortexed for 5 minutes and centrifuged at 4,000 rpm for 10 minutes (10 °C). 100 µL of supernatant was diluted in 100 µL of water. Final dilution factor was 6x.|
|Inj. Vol.:||2 µL|
|A:||Water + 0.1% formic acid|
|B:||Methanol + 0.1% formic acid|
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