Pure Chromatography
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Steroids in Serum on Raptor Biphenyl by LC-MS/MS
Conc. listed is the final concentration following sample preparation.

LC_CF0599
Column | Raptor Biphenyl (cat.# 9309A12) | ||||||||||||||||||||||||
Dimensions: | 100 mm x 2.1 mm ID | ||||||||||||||||||||||||
Particle Size: | 2.7 µm | ||||||||||||||||||||||||
Guard Column: | Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252) | ||||||||||||||||||||||||
Temp.: | 35 °C | ||||||||||||||||||||||||
Sample | |||||||||||||||||||||||||
Conc.: | 500 µL of mobile phase B was added to 250 µL of fortified beagle serum (100-400 ng/mL). The sample was vortexed for 5 minutes and centrifuged at 4,000 rpm for 10 minutes (10 °C). 100 µL of supernatant was diluted in 100 µL of water. Final dilution factor was 4x. | ||||||||||||||||||||||||
Inj. Vol.: | 2 µL | ||||||||||||||||||||||||
Mobile Phase | |||||||||||||||||||||||||
A: | Water + 0.1% formic acid | ||||||||||||||||||||||||
B: | Methanol + 0.1% formic acid | ||||||||||||||||||||||||
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Detector | MS/MS | ||||||||||||||||||||||||
Ion Mode: | ESI+ | ||||||||||||||||||||||||
Instrument | UHPLC |
RELATED CHROMATOGRAM LIBRARY SEARCHES
Androgen
,Progestagen
,Estrogen
,Mineralocorticoid
,Glucocorticoid
,Corticosteroid
,steroidogenesis
,Endocrine
,endochrinology
,Anabolic
,hormone
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