share:
delim
delim
Steroids in Serum on Raptor Biphenyl by LC-MS/MS
This chromatogram may contain analytes that are synonyms of your search terms.

Steroids in Serum on Raptor Biphenyl by LC-MS/MS

PeakstR (min)Conc.
(ng/mL)		Precursor IonProduct IonProduct Ion
1.Estriol1.44100289.1107.0253.2
2.Cortisol2.1150363.2121.0327.2
3.17β-Estradiol2.4550255.3159.1133.1
4.17α-Hydroxypregnenolone2.60100315.2297.2159.2
5.Aldosterone2.6850361.4315.2157.2
6.11-Deoxycortisol2.9550347.397.1109.1
7.Corticosterone3.0750347.4329.2121.1
8.Estrone3.0750271.2253.2133.1
9.Boldenone3.1125287.2121.0135.0
10.Epitestosterone3.1625289.2109.297.1
11.Dehydroepiandrosterone (DHEA)3.1650289.1253.1271.1
12.17α-hydroxyprogesterone3.3050331.297.0109.0
13.Testosterone3.3525289.297.0109.0
14.17β-trenbolone3.3650271.2253.1199.0
15.17α-Methyltestosterone3.4125303.297.0109.0
16.5α-Dihydrotestosterone (DHT)3.4150291.2255.2159.2
17.Nandrolone3.4250275.1109.0257.1
18.25-Hydroxy vitamin D33.71100383.3365.3257.2
19.Androstenedione3.7925287.197.0109.0
20.11-Deoxycorticosterone3.8125331.297.1109.1
21.Progesterone4.2125315.297.0109.0
Conc. listed is the final concentration following sample preparation.
image
LC_CF0599
ColumnRaptor Biphenyl (cat.# 9309A12)
Dimensions:100 mm x 2.1 mm ID
Particle Size:2.7 µm
Guard Column:Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)
Temp.:35 °C
Sample
Conc.: 500 µL of mobile phase B was added to 250 µL of fortified beagle serum (100-400 ng/mL). The sample was vortexed for 5 minutes and centrifuged at 4,000 rpm for 10 minutes (10 °C). 100 µL of supernatant was diluted in 100 µL of water. Final dilution factor was 4x.
Inj. Vol.:2 µL
Mobile Phase
A:Water + 0.1% formic acid
B:Methanol + 0.1% formic acid
Time (min)Flow (mL/min)%A%B
0.000.65050
4.000.60100
4.500.60100
4.510.65050
6.500.65050
DetectorMS/MS
Ion Mode:ESI+
InstrumentUHPLC
Download PDF

RELATED CHROMATOGRAM LIBRARY SEARCHES

Androgen

,

Progestagen

,

Estrogen

,

Mineralocorticoid

,

Glucocorticoid

,

Corticosteroid

,

steroidogenesis

,

Endocrine

,

endochrinology

,

Anabolic

,

hormone

Printing tips! To print chromatograms full page, change the print settings in the print dialogue window of your PDF reader software: 1) "fit" or "fit to page", 2) "landscape" for wide chromatograms or "portrait" for tall chromatograms.


Restek Domestic Customer Service

Subject

Message

Your Full Name

Your Email

Company Name

Address

Spam Block (Please leave this blank)

all fields required

Thank you

Your message has been sent. We will be in touch shortly.

Message not sent

Sorry, your message could not be sent at this time. Please try again later, or contact Restek or your local Restek representative via phone.

www.restek.com/Contact-Us