Pure Chromatography
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Nicotine-Related Compounds in Urine on Raptor Biphenyl by LC-MS/MS
| Peaks | tR (min) | Precursor Ion | Product Ion | |
|---|---|---|---|---|
| 1. | Nornicotine | 1.25 | 149.10 | 80.05 |
| 2. | Norcotinine | 1.81 | 163.09 | 80.05 |
| 3. | trans-3'-Hydroxycotinine | 1.88 | 193.12 | 80.05 |
| 4. | Nicotine | 1.95 | 163.15 | 132.10 |
| 5. | Anabasine | 2.20 | 163.13 | 91.63 |
| 6. | Cotinine | 2.88 | 177.12 | 98.07 |
LC_CF0606
| Column | Raptor Biphenyl (cat.# 9309512) | ||||||||||||||||||||||||||||
| Dimensions: | 100 mm x 2.1 mm ID | ||||||||||||||||||||||||||||
| Particle Size: | 5 µm | ||||||||||||||||||||||||||||
| Pore Size: | 90 Å | ||||||||||||||||||||||||||||
| Guard Column: | Raptor Biphenyl EXP cartridges 5 mm, 2.1 mm ID, 5 µm (cat.# 930950252) | ||||||||||||||||||||||||||||
| Temp.: | 30 °C | ||||||||||||||||||||||||||||
| Sample | |||||||||||||||||||||||||||||
| Diluent: | Water | ||||||||||||||||||||||||||||
| Conc.: | 25 ng/mL (fortified human urine) | ||||||||||||||||||||||||||||
| Inj. Vol.: | 5 µL | ||||||||||||||||||||||||||||
| Mobile Phase | |||||||||||||||||||||||||||||
| A: | 0.1% Formic acid, 5 mM ammonium formate in water | ||||||||||||||||||||||||||||
| B: | 0.1% Formic acid in methanol | ||||||||||||||||||||||||||||
| |||||||||||||||||||||||||||||
| Detector | MS/MS | ||||||||||||||||||||||||||||
| Ion Mode: | ESI+ | ||||||||||||||||||||||||||||
| Mode: | MRM | ||||||||||||||||||||||||||||
| Instrument | UHPLC | ||||||||||||||||||||||||||||
| Notes | The urine from a non-tobacco user was fortified with analytes to a final concentration of 25 ng/mL and prepared by a liquid-liquid extraction method. A 250 μL urine sample was mixed with 50 μL of 5N NaOH and extracted with 1.5 mL of 50/50 methylene chloride/diethyl ether. A portion of organic phase (1.0 mL) was dried and reconstituted with 200 μL of water for analysis. |
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Copyright © 2021 Restek Corporation. All rights reserved.
A Company of Owners
Cookies allow for a variety of features that make your visit to Restek more enjoyable. By using this website, you agree to the use of cookies in accordance with our guidelines. For more information, see our privacy policy.