Multiclass Drug Panel on Raptor Biphenyl by LC-MS/MS
Multiclass Drug Panel on Raptor Biphenyl by LC-MS/MS
Peaks
tR (min)
Conc. (ng/mL)
Precursor Ion
Product Ion
Product Ion
1.
trans-3-Hydroxycotinine
1.32
25
193.1
80.0
134.0
2.
Norcotinine
1.33
25
163.1
80.1
118.1
3.
Nicotine
1.40
25
163.2
130.1
117.1
4.
BZP
1.47
25
177.2
91.1
65.1
5.
Anabasine
1.53
25
163.2
120.1
80.1
6.
Noroxymorphone
1.78
25
288.1
270.1
212.9
7.
Lisdexamfetamine
1.79
25
264.2
84.1
91.0
8.
Phenelzine sulfate
1.90
25
137.2
105.1
77.1
9.
Acetaminophen
1.93
100
152.2
93.0
65.0
10.
Pregabalin
2.21
25
160.2
142.1
55.0
11.
Cotinine
2.27
25
177.2
80.0
98.1
12.
Morphine
2.27
25
286.2
152.1
165.0
13.
(+)-Ephedrine
2.37
25
166.2
148.1
115.0
14.
Oxymorphone
2.38
25
302.1
227.2
198.2
15.
Hydromorphone
2.61
25
286.2
184.9
156.9
16.
Amphetamine
2.63
25
136.2
91.0
65.1
17.
DMAA
2.65
25
116.1
57.1
99.2
18.
Gabapentin
2.71
25
172.2
154.0
137.1
19.
Methylephedrine
2.83
25
180.2
162.0
115.1
20.
Levetiracetam
2.84
25
171.1
125.9
69.2
21.
Desmethylolanzapine
2.94
25
299.1
256.1
198.0
22.
Norcodeine
2.96
25
286.1
151.9
115.0
23.
Olanzapine
3.12
25
313.2
256.1
198.1
24.
Methamphetamine
3.13
25
150.3
91.1
119.0
25.
MDA
3.18
25
180.1
163.1
105.2
26.
(-)-Naloxone
3.27
25
328.3
310.1
212.3
27.
Phentermine
3.29
25
150.2
91.1
133.1
28.
Noroxycodone
3.32
25
302.1
227.0
197.9
29.
Methylone
3.36
25
208.1
160.1
132.1
30.
O-Desmethyl-cis-tramadol
3.37
25
250.1
58.0
42.0
31.
(-)-Dihydrocodeine
3.38
25
302.2
199.0
128.2
32.
Norhydrocodone
3.43
25
286.1
199.0
128.2
33.
Codeine
3.43
25
300.2
152.0
165.1
34.
6-Acetylmorphine
3.47
25
328.2
165.0
211.0
35.
MDMA
3.60
25
194.2
163.0
135.1
36.
Desomorphine
3.61
25
272.1
152.1
165.0
37.
Oxycodone
3.64
25
316.2
298.0
169.0
38.
Naltrexone
3.71
25
342.2
324.0
267.0
39.
Levamisole
3.75
25
205.1
178.0
91.1
40.
Hydrocodone
3.76
25
300.1
199.0
128.0
41.
Diethylpropion
3.80
25
206.2
105.1
100.1
42.
Mephedrone
3.80
25
178.2
145.0
160.1
43.
2-Oxo-3-hydroxy-LSD
3.82
25
356.3
237.0
222.0
44.
o-Desmethylvenlafaxine
3.84
25
264.1
58.1
107.0
45.
6-β-Naltrexol
3.91
25
344.3
326.1
308.1
46.
Tapentadol-β-D-glucuronide
3.91
25
398.3
222.1
107.0
47.
7-Aminonitrazepam
3.95
25
252.2
121.1
94.2
48.
MDEA
4.00
25
208.1
163.0
105.2
49.
m-Hydroxybenzoylecgonine
4.02
25
306.1
168.1
121.0
50.
Lamotrigine
4.09
25
256.0
211.1
145.0
51.
Ritalinic acid
4.11
25
220.1
84.1
56.2
52.
N-Desmethyltapentadol
4.16
25
208.1
121.2
107.1
53.
Norbuprenorphine glucuronide
4.18
25
590.5
414.3
165.0
54.
Zonisamide
4.23
25
212.9
132.1
77.1
55.
mCPP
4.26
25
197.1
154.0
118.1
56.
Norketamine
4.28
25
224.1
125.0
89.1
57.
Nalbuphine
4.33
25
358.2
340.1
115.0
58.
Norfentanyl
4.33
25
233.1
84.1
55.0
59.
(+/-)-Hydroxybupropion
4.35
25
256.0
130.2
166.0
60.
Tapentadol
4.37
25
222.1
107.1
121.0
61.
Lacosamide
4.47
25
251.5
91.1
108.1
62.
Dextrorphan
4.50
25
258.2
157.0
201.2
63.
Levorphanol
4.50
25
258.2
156.8
199.2
64.
7-Hydroxyquetiapine
4.51
25
400.3
269.0
208.0
65.
Molindone
4.51
25
277.2
100.1
70.2
66.
Tramadol
4.52
25
264.2
58.0
77.1
67.
Zolpidem phenyl-4-carboxylic acid
4.60
25
338.2
265.1
219.0
68.
Felbamate
4.62
25
239.1
117.1
177.8
69.
N-Desmethyl-cis-tramadol
4.65
25
250.2
44.0
232.1
70.
Ketamine
4.66
25
238.1
125.1
89.1
71.
Benzoylecgonine
4.67
25
290.1
168.1
77.1
72.
Methylphenidate
4.68
25
234.1
84.1
56.1
73.
Normeperidine
4.76
25
234.1
160.2
91.0
74.
Meprobamate
4.79
25
219.1
158.2
97.0
75.
Meperidine
4.80
25
248.1
220.1
174.1
76.
Bupropion
4.81
25
240.0
184.1
130.2
77.
Heroin
4.85
25
370.4
328.2
164.9
78.
Cocaine
5.01
25
304.2
182.0
77.1
79.
7-Aminoclonazepam
5.02
25
286.1
121.2
250.1
80.
Zopiclone
5.04
25
389.2
245.0
217.2
81.
N-Desmethylmirtazapine
5.10
25
252.1
195.1
209.2
82.
Norcocaine
5.11
25
290.1
168.0
136.1
83.
Phenobarbital*
5.11
500
230.9
187.8
85.0
84.
Buprenorphine glucuronide
5.12
25
644.3
468.2
55.1
85.
MDPV
5.15
25
276.2
135.1
126.1
86.
Venlafaxine
5.15
25
278.4
260.4
121.2
87.
Milnacipran
5.24
25
247.2
100.1
129.1
88.
Pentazocine
5.29
25
286.2
218.1
69.2
89.
Mirtazapine
5.31
25
266.1
195.1
72.1
90.
Butalbital*
5.31
500
223.0
180.0
84.9
91.
Norbuprenorphine
5.35
50
414.3
152.2
165.2
92.
LSD
5.39
25
324.3
223.1
208.0
93.
Butorphanol
5.43
25
328.3
310.3
131.2
94.
Fosphenytoin
5.43
25
363.1
265.9
207.8
95.
Topiramate
5.44
25
340.2
264.2
223.9
96.
Cocaethylene
5.48
25
318.2
196.1
82.0
97.
Clozapine
5.48
25
328.2
271.1
193.1
98.
9-Hydroxyrisperidone
5.51
25
427.3
207.1
110.2
99.
Acetyl fentanyl
5.58
25
323.3
188.0
105.0
100.
Didesmethyl citalopram HCl
5.73
25
297.1
261.9
109.1
101.
7-Aminoflunitrazepam
5.75
25
284.1
135.0
227.1
102.
Amobarbital*
5.75
500
225.0
182.0
84.8
103.
Zolpidem
5.76
25
308.2
235.2
218.9
104.
N-Desmethylcitalopram
5.77
25
311.1
262.1
109.1
105.
Isocarboxazid
5.77
25
232.0
91.1
65.2
106.
Pentobarbital*
5.79
500
225.0
182.0
84.9
107.
Escitalopram
5.80
25
325.3
109.1
261.9
108.
Citalopram
5.80
25
325.2
109.1
262.0
109.
Diphenhydramine
5.80
25
256.1
167.0
152.1
110.
Desmethyldoxepin
5.88
25
266.1
107.1
115.0
111.
Ezogabine
5.91
25
304.0
230.1
109.1
112.
Doxepin
5.96
25
280.1
107.2
77.1
113.
Haloperidol
5.96
25
377.2
123.0
95.1
114.
Fluvoxamine
5.97
25
319.3
71.1
257.8
115.
Trazodone
5.99
25
372.3
176.1
148.0
116.
Norfluoxetine
6.01
25
296.3
134.3
104.9
117.
Fentanyl
6.02
25
337.3
188.0
105.1
118.
Oxcarbazepine
6.02
25
253.1
180.0
208.1
119.
Risperidone
6.03
25
411.2
191.0
110.1
120.
Buprenorphine
6.04
50
468.3
55.1
414.2
121.
Fluoxetine
6.05
25
310.1
148.0
115.1
122.
Secobarbital*
6.08
500
237.0
193.9
84.8
123.
Phenytoin
6.12
25
253.0
103.9
181.7
124.
Carisoprodol
6.13
25
261.1
176.0
62.0
125.
Flurazepam
6.14
25
388.2
315.2
183.0
126.
Chlordiazepoxide
6.14
25
300.1
282.0
227.0
127.
Dextromethorphan
6.16
25
272.2
215.1
170.9
128.
PCP
6.18
25
244.2
86.1
159.1
129.
Tenoxicam
6.18
25
338.1
121.0
95.0
130.
Buspirone
6.20
25
386.3
122.1
95.0
131.
Quetiapine
6.28
25
384.2
253.0
221.2
132.
Norpropoxyphene
6.29
25
326.4
252.0
91.1
133.
Propoxyphene
6.30
25
340.3
265.9
58.1
134.
Asenapine
6.35
25
286.2
165.1
229.1
135.
Ziprasidone
6.40
25
413.2
194.1
130.0
136.
Protriptyline
6.42
25
264.1
191.1
165.2
137.
Promazine
6.42
25
285.1
58.0
86.0
138.
Midazolam
6.43
25
326.2
291.1
248.9
139.
Desipramine
6.43
25
267.1
72.1
193.1
140.
Amoxapine
6.44
25
314.2
271.0
193.1
141.
Sufentanil
6.48
25
387.2
238.1
111.1
142.
Imipramine
6.51
25
281.1
86.2
58.1
143.
Paroxetine
6.51
25
330.2
192.2
70.1
144.
Loxapine
6.52
25
328.1
271.1
193.0
145.
Maprotiline
6.52
25
278.1
250.2
191.0
146.
Duloxetine
6.52
25
298.1
154.1
188.2
147.
Metaxalone
6.52
25
222.1
161.1
77.1
148.
Carbamazepine
6.55
10
237.1
194.1
165.0
149.
Cyclobenzaprine
6.56
25
276.2
215.0
189.0
150.
EDDP
6.57
25
278.2
234.3
249.2
151.
Nortriptyline
6.58
25
264.1
91.1
115.2
152.
Mitragynine
6.59
25
399.3
174.2
159.0
153.
Eslicarbazepine
6.60
25
297.2
194.0
237.1
154.
Amitriptyline
6.65
25
278.1
91.1
202.1
155.
α-Hydroxymidazolam
6.70
25
342.2
324.1
202.9
156.
Trimipramine
6.75
25
295.2
100.2
58.2
157.
N-Desmethylflunitrazepam
6.80
25
300.1
254.1
198.1
158.
Lorazepam
6.82
25
321.1
275.0
229.0
159.
Mesoridazine
6.83
25
387.2
126.1
98.2
160.
Tiagabine
6.89
25
376.2
246.8
111.1
161.
Ketorolac
6.90
25
256.1
104.9
77.0
162.
Chlorpromazine
6.91
25
320.1
58.1
86.1
163.
Loxoprofen*
6.93
200
245.0
83.2
80.9
164.
Clomipramine
6.95
25
315.3
86.0
58.0
165.
Oxazepam
6.96
25
287.1
241.2
268.8
166.
Methadone
6.98
25
310.2
264.9
105.1
167.
Sertraline
6.98
25
306.2
158.9
275.1
168.
Dehydro aripiprazole
7.01
25
446.2
285.0
98.1
169.
Clonazepam
7.01
25
316.1
270.0
214.1
170.
Nitrazepam
7.01
25
282.1
235.9
180.1
171.
Lornoxicam
7.02
100
373.1
121.1
95.0
172.
25I-NBOMe
7.05
25
428.1
121.2
91.1
173.
Salsalate*
7.05
100
256.8
136.8
92.9
174.
Desalkylflurazepam
7.09
25
289.0
140.1
104.0
175.
Tolmetin
7.10
25
258.1
119.1
91.0
176.
α-Hydroxytriazolam
7.12
25
359.1
330.9
175.9
177.
Fluphenazine
7.16
25
438.3
171.1
143.2
178.
2-Hydroxyethylflurazepam
7.16
25
333.1
211.1
109.0
179.
Ketoprofen*
7.17
100
253.0
209.0
-
180.
Naproxen*
7.17
200
229.0
168.9
184.9
181.
EMDP
7.19
25
264.2
235.2
220.3
182.
Aripiprazole
7.20
25
448.2
285.0
176.1
183.
Perphenazine
7.22
25
404.2
171.1
143.2
184.
Etoricoxib
7.24
25
359.2
280.0
279.2
185.
Methaqualone
7.24
25
251.1
91.1
132.2
186.
α-Hydroxyalprazolam
7.25
25
325.1
297.0
216.2
187.
Trifluoperazine
7.28
25
408.2
141.2
113.1
188.
Piroxicam
7.34
25
332.1
95.2
78.1
189.
Ibuprofen*
7.34
200
205.0
160.9
-
190.
Prochlorperazine
7.36
25
374.1
141.0
113.1
191.
Etodolac*
7.36
50
286.0
241.9
212.0
192.
Nordiazepam
7.37
25
271.0
139.9
208.0
193.
Phenazepam
7.39
25
349.1
206.2
179.0
194.
Fenoprofen*
7.40
200
240.9
92.8
-
195.
Zaleplon
7.43
25
306.1
264.0
236.0
196.
Clobazam
7.43
25
301.0
259.1
224.0
197.
Flurbiprofen*
7.48
200
243.0
199.0
196.8
198.
Thiothixene
7.50
25
444.2
221.2
235.0
199.
Flunitrazepam
7.55
25
314.2
267.9
239.1
200.
Meloxicam
7.55
25
352.1
115.0
141.0
201.
Triazolam
7.58
25
343.1
307.9
315.0
202.
Estazolam
7.58
25
295.1
266.9
205.1
203.
Celecoxib
7.60
25
382.2
362.0
300.1
204.
Parecoxib
7.60
25
371.2
234.0
165.1
205.
Temazepam
7.61
25
301.1
255.1
282.9
206.
Sulindac
7.70
25
357.2
233.1
248.0
207.
Alprazolam
7.72
25
309.1
280.9
204.9
208.
Flufenamic acid*
7.75
50
279.9
236.1
176.2
209.
Thioridazine
7.76
25
371.2
126.1
98.1
210.
Diclofenac*
7.78
100
293.9
249.8
-
211.
Nabumetone
7.80
25
229.1
171.1
128.1
212.
Oxaprozin
7.87
25
294.1
103.0
234.2
213.
HU-211
8.03
25
387.3
155.9
128.1
214.
JWH‐200
8.04
25
385.3
155.0
127.1
215.
Diazepam
8.04
25
285.1
192.9
153.9
216.
Mefenamic acid
8.04
25
242.1
224.1
-
217.
Tolfenamic acid*
8.17
50
259.9
215.8
-
218.
Meclofenamic acid*
8.18
200
293.9
257.8
177.7
219.
(+/-)-CP 47,497*
8.27
200
317.1
245.1
159.0
220.
Indomethacin*
8.29
50
356.0
311.8
296.9
221.
Perampanel
8.32
25
350.2
219.1
247.1
222.
(+/-)-CP 47,497 C8 homologue*
8.42
200
331.1
259.0
159.1
223.
11-Nor-9-carboxy-delta-9-THC*
8.44
25
343.0
298.9
244.8
224.
Prazepam
8.51
25
325.2
271.0
139.9
225.
XLR-11
8.60
25
330.3
125.2
144.0
226.
Δ9-THC
8.76
25
315.2
193.0
123.2
227.
UR-144
8.78
25
312.3
125.1
214.1
228.
JWH-250
8.96
25
336.3
120.9
91.1
229.
THCA-A*
9.12
25
357.1
312.9
245.0
230.
JWH-073
9.21
25
328.2
155.0
127.1
231.
JWH‐018
9.35
25
342.3
127.1
155.1
*Analyzed using negative polarity.
LC_CF0621
Column
Raptor Biphenyl (cat.# 9309A12)
Dimensions:
100 mm x 2.1 mm ID
Particle Size:
2.7 µm
Guard Column:
Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252)
Temp.:
30 °C
Sample
Diluent:
Water
Inj. Vol.:
5 µL
Mobile Phase
A:
Water + 0.1% formic acid + 2 mM ammonium formate
B:
Methanol + 0.1% formic acid + 2 mM ammonium formate
Time (min)
Flow (mL/min)
%A
%B
0.00
0.6
95
5
9.00
0.6
0
100
10.00
0.6
0
100
10.01
0.6
95
5
12.00
0.6
95
5
Detector
MS/MS
Ion Mode:
ESI+/ESI-
Mode:
Scheduled MRM
Instrument
UHPLC
Notes
1. Positive and negative polarity data were collected simultaneously from a single injection. 2. Ibuprofen, fosphenytoin, and topiramate degrade quickly in solution. 3. Levorphanol and dextrorphan are enantiomers and are not resolved. 4. Escitalopram is a pure enantiomer and citalopram is a racemic mixture of both enantiomers. These compounds are not resolved. 5. DMAA consists of 4 stereoisomers, 2 of which are partially resolved. 6. Doxepin and desmethylydoxepin consist of a mixture of cis/trans isomers. The isomers are fully resolved and the retention time identifies the main isomer component. 7. Lornoxicam, meloxicam, etodolac, oxaprozin, indomethacin, and mefenamic acid can be detected by positive or negative polarity. The polarity chosen in this chromatogram provides optimal response.
Printing tips! To print chromatograms full page, change the print settings in the print dialogue window of your PDF reader software: 1) "fit" or "fit to page", 2) "landscape" for wide chromatograms or "portrait" for tall chromatograms.
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