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Synthetic and Naturally Occurring Glucocorticoids on Raptor Biphenyl by LC-MS/MS

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Synthetic and Naturally Occurring Glucocorticoids on Raptor Biphenyl by LC-MS/MS

	Peaks	t_R (min)	Precursor Ion	Product Ion	Product Ion
1.	Triamcinolone	1.19	395.1	357.1	91.2
2.	Prednisolone	1.91	361.2	343.2	325.1
3.	Cortisol	1.95	363.1	121.2	91.1
4.	Prednisone	2.08	359.1	341.1	147.1
5.	Cortisone	2.15	361.1	163.1	91.2
6.	Methylprednisolone	2.30	375.1	357.1	91.1
7.	Betamethasone	2.40	393.1	373.1	91.2
8.	Dexamethasone	2.44	393.1	373.1	355.2
9.	Flumethasone	2.49	411.1	121.1	91.2
10.	Beclomethasone	2.72	409.2	391.2	147.2
11.	11-Deoxycortisol	2.96	347.1	109.1	97.0
12.	Budesonide isomers*	3.35	431.1	413.2	147.1
13.	Fluticasone propionate	3.57	501.0	313.1	293.2

*Retention time listed is for the first eluting isomer.

LC_CF0658

Column

Raptor Biphenyl (cat.# 9309A12)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Temp.:

40 °C

Sample

Diluent:

Water:methanol (60:40)

Conc.:

20 ng/mL

Inj. Vol.:

5 µL

Mobile Phase

Water + 0.1% formic acid

Acetonitrile + 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.5	75	25
2.00	0.5	60	40
2.50	0.5	60	40
3.00	0.5	0	100
3.75	0.5	0	100
3.76	0.5	75	25
5.50	0.5	75	25

Detector

MS/MS

Ion Mode:

ESI+

Mode:

MRM

Instrument

UHPLC

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Synthetic and Naturally Occurring Glucocorticoids on Raptor Biphenyl by LC-MS/MS

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