Pure Chromatography
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Immunosuppressive Drugs in Blood on Raptor Biphenyl by LC-MS/MS

LC_CF0686
Column | Raptor Biphenyl (cat.# 9309A52) | ||||||||||||||||||||
Dimensions: | 50 mm x 2.1 mm ID | ||||||||||||||||||||
Particle Size: | 2.7 µm | ||||||||||||||||||||
Pore Size: | 90 Å | ||||||||||||||||||||
Guard Column: | Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9309A0252) | ||||||||||||||||||||
Temp.: | 70 °C | ||||||||||||||||||||
Inj. Vol.: | 5 µL | ||||||||||||||||||||
Mobile Phase | |||||||||||||||||||||
A: | 0.05% Formic acid, 5 mM ammonium formate in water | ||||||||||||||||||||
B: | Methanol | ||||||||||||||||||||
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Max Pressure: | 305 bar | ||||||||||||||||||||
Detector | MS/MS | ||||||||||||||||||||
Ion Mode: | ESI+ | ||||||||||||||||||||
Mode: | MRM | ||||||||||||||||||||
Instrument | UHPLC | ||||||||||||||||||||
Notes | Sample Preparation Procedure: Human whole blood was fortified with 4 immunosuppressive drugs at 10 ng/mL. For quantitation, cyclosporin D was used as the internal standard for cyclosporin A and ascomycin was used as the internal standard for tacrolimus, sirolimus, and everolimus. The blood sample (100 μL) was mixed with 200 μL of precipitation solution (1:4 v/v 0.2 M ZnSO4:methanol) containing 50 ng/mL of cyclosporin D and 5 ng/mL of ascomycin. The mixture was vortexed for 20 seconds at 3,000 rpm and then centrifuged for 10 minutes at 4,300 rpm. The supernatant was directly injected (5 μL) for analysis. |
RELATED CHROMATOGRAM LIBRARY SEARCHES
cyclosporin
,tacrolimus
,sirolimus
,everolimus
,immunosuppressive
,immunosuppresant
,raptor biphenyl
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