share:
delim
delim
Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine on Raptor EtG/EtS

Ethyl Glucuronide (EtG) and Ethyl Sulfate (EtS) in Urine on Raptor EtG/EtS

PeakstR (min)Precursor IonProduct IonProduct Ion
1.Ethyl-β-D-glucuronide-d50.86225.984.9-
2.Ethyl-β-D-glucuronide0.87220.884.974.8
3.Ethyl sulfate-d52.14129.797.7-
4.Ethyl sulfate2.17124.796.879.7
image
LC_CF0704
ColumnRaptor EtG/EtS (cat.# 9325A12)
Dimensions:100 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Guard Column:UltraShield UHPLC precolumn filter, 0.2 µm frit (cat.# 25809)
Temp.:35 °C
Sample
Diluent:0.1% Formic acid in water
Conc.: A 500 ng/mL standard was prepared in urine. 50 µL of the standard was diluted with 950 µL of a working internal standard (25 ng/mL EtS-d5/100 ng/mL EtG-d5 in 0.1% formic acid in water). The sample was vortexed at 3,500 rpm for 10 seconds to mix. The sample was then centrifuged at 3,000 rpm for 5 minutes at 10 °C. The autosampler needle was adjusted to inject from the supernatant.
Inj. Vol.:10 µL
Mobile Phase
A:0.1% Formic acid in water
B:0.1% Formic acid in acetonitrile
Time (min)Flow (mL/min)%A%B
0.000.5955
2.500.56535
2.510.5955
4.000.5955
DetectorMS/MS
Ion Mode:ESI-
Mode:MRM
InstrumentHPLC
NotesReference Standards
Ethyl-β-D-glucuronide (cat.# 34101)
Ethyl-β-D-glucuronide-d5 (cat.# 34102)
Ethyl sulfate sodium salt (cat.# 34103)
Ethyl sulfate-d5 sodium salt (cat.# 34104)
Download PDF

RELATED CHROMATOGRAM LIBRARY SEARCHES

EtG

,

EtS

Printing tips! To print chromatograms full page, change the print settings in the print dialogue window of your PDF reader software: 1) "fit" or "fit to page", 2) "landscape" for wide chromatograms or "portrait" for tall chromatograms.


Restek Domestic Customer Service

Subject

Message

Your Full Name

Your Email

Company Name

Address

Spam Block (Please leave this blank)

all fields required

Thank you

Your message has been sent. We will be in touch shortly.

Message not sent

Sorry, your message could not be sent at this time. Please try again later, or contact Restek or your local Restek representative via phone.

www.restek.com/Contact-Us