Pure Chromatography
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This chromatogram may contain analytes that are synonyms of your search terms.
Fentanyl and Analogues in Urine on Raptor Biphenyl by LC-MS/MS
| Peaks | tR (min) | Conc. (ng/mL) | Precursor Ion | Product Ion | Product Ion | |
|---|---|---|---|---|---|---|
| 1. | Norfentanyl-d5 | 1.03 | 1 | 238.30 | 84.15 | - |
| 2. | Norfentanyl | 1.04 | 10 | 233.27 | 84.15 | 56.06 |
| 3. | Remifentanil | 1.34 | 10 | 377.37 | 113.15 | 317.30 |
| 4. | Acetyl fentanyl-13C6 | 1.69 | 1 | 329.37 | 188.25 | - |
| 5. | Acetyl fentanyl | 1.69 | 10 | 323.37 | 188.25 | 105.15 |
| 6. | Alfentanil | 1.88 | 10 | 417.47 | 268.31 | 197.23 |
| 7. | Fentanyl-d5 | 1.95 | 1 | 342.47 | 188.27 | - |
| 8. | Fentanyl | 1.96 | 10 | 337.37 | 188.26 | 105.08 |
| 9. | Carfentanil-d5 | 2.04 | 1 | 400.40 | 340.41 | - |
| 10. | Carfentanil | 2.05 | 10 | 395.40 | 113.14 | 335.35 |
| 11. | Butyryl fentanyl | 2.15 | 10 | 351.43 | 188.20 | 105.15 |
| 12. | Sufentanil-d5 | 2.25 | 1 | 392.40 | 238.25 | - |
| 13. | Sufentanil | 2.26 | 10 | 387.40 | 238.19 | 111.06 |
LC_CF0715
| Column | Raptor Biphenyl (cat.# 9309552) | ||||||||||||||||||||
| Dimensions: | 50 mm x 2.1 mm ID | ||||||||||||||||||||
| Particle Size: | 5 µm | ||||||||||||||||||||
| Pore Size: | 90 Å | ||||||||||||||||||||
| Guard Column: | Raptor Biphenyl EXP guard cartridge 5 mm, 2.1 mm ID, 5 µm (cat.# 930950252) | ||||||||||||||||||||
| Temp.: | 40 °C | ||||||||||||||||||||
| Sample | |||||||||||||||||||||
| Diluent: | 70:30 Water:methanol | ||||||||||||||||||||
| Inj. Vol.: | 5 µL | ||||||||||||||||||||
| Mobile Phase | |||||||||||||||||||||
| A: | 0.1% Formic acid in water | ||||||||||||||||||||
| B: | 0.1% Formic acid in methanol | ||||||||||||||||||||
| |||||||||||||||||||||
| Detector | MS/MS | ||||||||||||||||||||
| Ion Mode: | ESI+ | ||||||||||||||||||||
| Mode: | MRM | ||||||||||||||||||||
| Instrument | UHPLC | ||||||||||||||||||||
| Notes | Human urine was fortified at 10 ng/mL with target analytes. An 80 μL urine aliquot was mixed with 320 μL of 70:30 water:methanol solution (5-fold dilution) and 10 μL of internal standard solution (40 ng/mL in methanol) in a Thomson SINGLE StEP filter vial (Restek cat.# 25895). After filtering through the 0.2 µm PVDF membrane, 5 μL was injected for analysis. |
RELATED CHROMATOGRAM LIBRARY SEARCHES
alfentanil
,remifentanil
,carfentanil
,butyryl fentanyl
,fentanyl
,norfentanyl
,sufentani
,acetyl fentanyl
,Raptor
,biphenyl
,LC-MS/MS
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Restek Corporation, U.S., 110 Benner Circle, Bellefonte, PA 16823
Copyright © 2021 Restek Corporation. All rights reserved.
A Company of Owners
Cookies allow for a variety of features that make your visit to Restek more enjoyable. By using this website, you agree to the use of cookies in accordance with our guidelines. For more information, see our privacy policy.