Pure Chromatography
www.restek.com
www.restek.com



This chromatogram may contain analytes that are synonyms of your search terms.
Creatine and Creatinine in Human Plasma on Raptor HILIC-Si by LC-MS/MS
Peaks | tR (min) | Precursor Ion | Product Ion | Product Ion | |
---|---|---|---|---|---|
1. | Creatinine![]() | 0.537 | 114.0 | 44.3 | 86.0 |
2. | Creatine![]() | 1.232 | 132.1 | 43.3 | 90.2 |

LC_CF0722
Column | Raptor HILIC-Si (cat.# 9310A52) | |||||||||
Dimensions: | 50 mm x 2.1 mm ID | |||||||||
Particle Size: | 2.7 µm | |||||||||
Pore Size: | 90 Å | |||||||||
Guard Column: | UltraShield UHPLC precolumn filter 0.2 µm (cat.# 25810) | |||||||||
Temp.: | 40 °C | |||||||||
Sample | ||||||||||
Diluent: | 20:80 Water:acetonitrile | |||||||||
Conc.: | Endogenous levels | |||||||||
Inj. Vol.: | 0.2 µL | |||||||||
Mobile Phase | ||||||||||
A: | 5 mM Ammonium formate in 20:80 water:acetonitrile | |||||||||
| ||||||||||
Detector | MS/MS | |||||||||
Ion Mode: | ESI+ | |||||||||
Mode: | MRM | |||||||||
Instrument | UHPLC | |||||||||
Notes | Sample Preparation: Endogenous levels of creatinine and creatine in human plasma were determined using a single protein precipitation step followed by LC-MS/MS analysis. A 50 μL aliquot of human plasma (K2EDTA) was mixed with 950 μL acetonitrile. After vortexing and centrifuging at 4,300 rpm for 10 min, 200 μL of the supernatant was transferred to a new vial and mixed with 50 μL of water. Centrifugation was performed again before injection. |
RELATED CHROMATOGRAM LIBRARY SEARCHES
Printing tips! To print chromatograms full page, change the print settings in the print dialogue window of your PDF reader software: 1) "fit" or "fit to page", 2) "landscape" for wide chromatograms or "portrait" for tall chromatograms.