Pure Chromatography
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Creatine and Creatinine in Human Plasma on Raptor HILIC-Si by LC-MS/MS
| Peaks | tR (min) | Precursor Ion | Product Ion | Product Ion | |
|---|---|---|---|---|---|
| 1. | Creatinine | 0.537 | 114.0 | 44.3 | 86.0 |
| 2. | Creatine | 1.232 | 132.1 | 43.3 | 90.2 |
LC_CF0722
| Column | Raptor HILIC-Si (cat.# 9310A52) | |||||||||
| Dimensions: | 50 mm x 2.1 mm ID | |||||||||
| Particle Size: | 2.7 µm | |||||||||
| Pore Size: | 90 Å | |||||||||
| Guard Column: | UltraShield UHPLC precolumn filter 0.2 µm (cat.# 25810) | |||||||||
| Temp.: | 40 °C | |||||||||
| Sample | ||||||||||
| Diluent: | 20:80 Water:acetonitrile | |||||||||
| Conc.: | Endogenous levels | |||||||||
| Inj. Vol.: | 0.2 µL | |||||||||
| Mobile Phase | ||||||||||
| A: | 5 mM Ammonium formate in 20:80 water:acetonitrile | |||||||||
| ||||||||||
| Detector | MS/MS | |||||||||
| Ion Mode: | ESI+ | |||||||||
| Mode: | MRM | |||||||||
| Instrument | UHPLC | |||||||||
| Notes | Sample Preparation: Endogenous levels of creatinine and creatine in human plasma were determined using a single protein precipitation step followed by LC-MS/MS analysis. A 50 μL aliquot of human plasma (K2EDTA) was mixed with 950 μL acetonitrile. After vortexing and centrifuging at 4,300 rpm for 10 min, 200 μL of the supernatant was transferred to a new vial and mixed with 50 μL of water. Centrifugation was performed again before injection. |
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Restek Corporation, U.S., 110 Benner Circle, Bellefonte, PA 16823
Copyright © 2020 Restek Corporation. All rights reserved.
A Company of Owners
Cookies allow for a variety of features that make your visit to Restek more enjoyable. By using this website, you agree to the use of cookies in accordance with our guidelines. For more information, see our privacy policy.