GHB and Related Compounds in Human Blood by LC-MS/MS

	Peaks	t_R (min)	Precursor ion	Product ion
1.	γ-Hydroxybutyric acid (GHB)	1.42	105.2	87.0
2.	1,4-Butanediol (1,4-BD)	1.42	91.0	55.0
3.	γ-Butyrolactone (GBL)	1.75	87.0	45.0

LC_CF0732

Column

Force C18 (cat.# 963431E)

Dimensions:

100 mm x 3.0 mm ID

Particle Size:

3 µm

Pore Size:

100 Å

Guard Column:

Force C18 EXP guard column cartridge 5 mm, 3.0 mm ID, 3 µm (cat.# 963450253)

Temp.:

30 °C

Sample

Diluent:

Water

Conc.:

500 ng/mL

Inj. Vol.:

10 µL

Mobile Phase

0.5% Formic acid in water

0.5% Formic acid in methanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.7	95	5
0.50	0.7	95	5
3.00	0.7	50	50
3.01	0.7	95	5
5.00	0.7	95	5

Detector

MS/MS

Ion Mode:

ESI+

Mode:

MRM

Instrument

HPLC

Notes

100 μL of whole human blood was fortified at 50 μg/mL with GHB, GBL, 1,4-BD, and GHB-D6 (IS) using 5 μL of 1 mg/mL solutions. The blood was precipitated with 380 μL methanol. The sample was then vortexed at 1000 rpm for 10 seconds and centrifuged at 3000 rpm for 10 minutes at 10 °C. 50 μL of the supernatant was removed and diluted to 1 mL with water. The sample was then vortexed and subjected to LC-MS/MS analysis. (Internal standard not shown on chromatogram.)

Download PDF

GHB

GBL

Printing tips! To print chromatograms full page, change the print settings in the print dialogue window of your PDF reader software: 1) "fit" or "fit to page", 2) "landscape" for wide chromatograms or "portrait" for tall chromatograms.

More Search Options

GHB and Related Compounds in Human Blood by LC-MS/MS

RELATED CHROMATOGRAM LIBRARY SEARCHES

GHB

GBL

Restek Domestic Customer Service

Thank you

Message not sent

	Force EXP Guard Column Cartridges cat.# 963450253 Qty:
	Force C18 LC Columns cat.# 963431E Qty: