Phosphatidylethanol 16:0/18:1 in Human Whole Blood on Raptor FluoroPhenyl

	Peaks	t_R (min)	Conc.	Precursor Ion	Product Ion
1.	Phosphatidylethanol-d5 (PEth-d5) 16:0/18:1	1.58	0.4 μM	706.6	281.3
2.	Phosphatidylethanol (PEth) 16:0/18:1	1.58	0.5 μM	701.5	255.3

LC_CF0733

Column

Raptor FluoroPhenyl (cat.# 9319A52)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Temp.:

40 °C

Sample

Conc.:

0.5 μM in blood

Inj. Vol.:

2 µL

Mobile Phase

Water, 5 mM ammonium acetate

9:1 Methanol:2-propanol

Time (min)	Flow (mL/min)	%A	%B
0.00	0.5	30	70
1.00	0.5	20	80
2.00	0.5	20	80
2.50	0.5	0	100
2.51	0.5	30	70
3.50	0.5	30	70

Detector

MS/MS

Ion Mode:

ESI-

Mode:

MRM

Instrument

UHPLC

Notes

Sample Preparation Procedure (Provided by Redhot Diagnostics):
The fortified blood sample (50 µL) was mixed with 50 µL of internal standard (0.4 µM PEth-d5 in 2-propanol) and 150 µL of 4:1 2-propanol:tetrahydrofuran. The mixture was vortexed for 20 seconds at 3000 rpm and centrifuged for 10 minutes at 4300 rpm. The supernatant (2 µL) was injected for analysis.

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PEth

phosphatidylethanol

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Phosphatidylethanol 16:0/18:1 in Human Whole Blood on Raptor FluoroPhenyl

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