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22 Acylcarnitines and 13 Amino Acids in Solvent on Raptor HILIC-Si EXP Guard Column by LC-MS/MS

22 Acylcarnitines and 13 Amino Acids in Solvent on Raptor HILIC-Si EXP Guard Column by LC-MS/MS

PeakstR (min)Precursor IonProduct Ion
1.Phenylalanine0.20166.0120.1
2.Leucine0.21132.186.0
3.Isoleucine0.21132.186.1
4.Tyrosine0.22182.191.0
5.Methionine0.24150.156.1
6.Valine0.27118.172.0
7.C20-Eicosanoyl-L-carnitine0.27456.485.1
8.C18-Stearoyl-L-carnitine0.28428.385.1
9.C18:1 Oleoyl-L-carnitine0.29426.485.1
10.C18:2 Linoleoyl-L-carnitine0.30424.385.1
11.C16-Palmitoyl-L-carnitine0.30400.385.1
12.C16:1 Palmitolelyl-L-carnitine0.31398.385.1
13.C14-Myristoyl-L-carnitine0.32372.385.1
14.C14:1 Tertradecenoyl-L-carnitine0.33370.385.1
15.C14:2-Tetradecadienoyl-L-carnitine0.33368.385.1
16.Proline0.33116.070.1
17.C12-Lauroyl-L-carnitine0.35344.385.1
18.Alanine0.3690.144.1
19.C10-Decanoyl-L-carnitine0.38316.385.1
20.C8-Octanoyl-L-carnitine0.43288.385.1
21.C7-Heptanoyl-L-carnitine0.45274.285.1
22.C6-Hexanoyl-L-carnitine0.47260.285.1
23.Glutamine0.45147.184.1
24.C5-Valeryl-L-carnitine0.48246.285.1
25.C5-Isovaleryl-L-carnitine0.49246.185.1
26.2-Methylbutyryl-L-carnitine0.49246.285.1
27.C5:1-Tiglyl-L-carnitine0.50244.285.1
28.C4-Butyryl-L-carnitine0.51232.285.1
29.C4-Isobutyryl-L-carnitine0.51232.185.1
30.Citrulline0.51176.1113.1
31.Glutamic acid0.55148.183.9
32.C3-Propionyl-L-carnitine0.54218.185.1
33.C2-Acetyl-L-carnitine0.56204.185.1
34.Arginine0.66175.270.1
35.Ornithine0.69133.170.1
Internal standard peaks not shown.
image
LC_CF0758
ColumnRaptor HILIC-Si EXP guard column cartridge (cat.# 9310A0252)
Dimensions:5 mm x 2.1 mm ID
Particle Size:2.7 µm
Temp.:45 °C
Sample
Diluent:85:15 Acetonitrile:water (v/v)
Conc.:100 ng/mL
Inj. Vol.:2 µL
Mobile Phase
A:30 mM Ammonium formate in water
B:Acetonitrile
Time (min)Flow (mL/min)%A%B
0.000.51585
0.40.57030
0.410.51585
1.200.51585
DetectorMS/MS
Ion Source:Electrospray
Ion Mode:ESI+
Instrument4500
NotesA 100 ng/mL standard mix of 22 acylcarnitines and 13 amino acids was prepared in 85:15 acetonitrile:water (v/v) which was fortified with known concentrations of stable isotope labeled internal standards. The solution was vortexed at 3000 rpm for 10 seconds to mix, and then the supernatant was injected for LC-MS/MS analysis using a Raptor HILIC-Si EXP guard column (no analytical column). The total cycle time was 1.2 minutes.
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Acylcarnitines

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