Pure Chromatography
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22 Acylcarnitines and 13 Amino Acids in Solvent on Raptor HILIC-Si EXP Guard Cartridge Column by LC-MS/MS
Internal standard peaks not shown.

LC_CF0758
Column | Raptor HILIC-Si EXP guard cartridge column (cat.# 9310A0252) | ||||||||||||||||||||
Dimensions: | 5 mm x 2.1 mm ID | ||||||||||||||||||||
Particle Size: | 2.7 µm | ||||||||||||||||||||
Temp.: | 45 °C | ||||||||||||||||||||
Sample | |||||||||||||||||||||
Diluent: | 85:15 Acetonitrile:water (v/v) | ||||||||||||||||||||
Conc.: | 100 ng/mL | ||||||||||||||||||||
Inj. Vol.: | 2 µL | ||||||||||||||||||||
Mobile Phase | |||||||||||||||||||||
A: | 30 mM Ammonium formate in water | ||||||||||||||||||||
B: | Acetonitrile | ||||||||||||||||||||
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Detector | MS/MS | ||||||||||||||||||||
Ion Source: | Electrospray | ||||||||||||||||||||
Ion Mode: | ESI+ | ||||||||||||||||||||
Instrument | 4500 | ||||||||||||||||||||
Notes | A 100 ng/mL standard mix of 22 acylcarnitines and 13 amino acids was prepared in 85:15 acetonitrile:water (v/v) that was fortified with known concentrations of stable isotope-labeled internal standards. The solution was vortexed at 3000 rpm for 10 seconds to mix, and then the supernatant was injected for LC-MS/MS analysis. |
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