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22 Acylcarnitines and 13 Amino acids (Endogenous) in Dried Blood Spots with 16 Deuterated Internal Standards on Raptor HILIC-Si EXP Guard Cartridge Column by LC-MS/MS
All analytes were present endogenously at varying concentrations in whole blood, except the deuterated internal standards.
|Column||Raptor HILIC-Si EXP guard cartridge column (cat.# 9310A0252)|
|Dimensions:||5 mm x 2.1 mm ID|
|Particle Size:||2.7 µm|
|Diluent:||85:15 Acetonitrile:water (v/v)|
|Inj. Vol.:||2.0 µL|
|A:||30 mM Ammonium formate in water|
|Notes||50 µL of whole blood was spotted on to Whatman 903 neonatal protein saver cards, which were then dried for 1 hour at room temperature. A 3.0 mm disk (∼3.0 µL whole blood) was punched out of the dried spot and into a 2.0 mL Eppendorf tube. 200 µL of 85:15 acetonitrile:water (v/v) that was fortified with known concentrations of stable isotope-labeled internal standards was added, and then the sample was vortexed and incubated for 20 minutes at room temperature on a microplate shaker at a speed of 400 rpm. The sample was then centrifuged for 10 minutes at 4000 rpm, and 150 µL of the supernatant was filtered using a Thomson SINGLE StEP Nano filter vial (cat.# 25882) prior to LC-MS/MS analysis.|
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