Pure Chromatography
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Incurred Pesticides in Red Bell Pepper QuEChERS Extract on Ultra Aqueous C18
Peaks | |
---|---|
1. | Methamidophos![]() |
2. | Omethoate![]() |
3. | Thiamethoxam![]() |
4. | Dimethoate![]() |
5. | Clothianidin![]() |
6. | Imidacloprid![]() |
7. | Propamocarb![]() |
8. | Carbaryl![]() |
9. | Metalaxyl![]() |
10. | Diphenylamine![]() |
11. | Myclobutanil![]() |
12. | Spinosyn A |
13. | Spinosyn D |

LC_FF0515
Column | Ultra Aqueous C18 (cat.# 9178312) |
Dimensions: | 100 mm x 2.1 mm ID |
Particle Size: | 3 µm |
Pore Size: | 100 Å |
Sample | QuEChERS extract of red bell pepper |
Nicarbazin (bis-nitrophenol urea) (cat.# 33261) | |
Diluent: | Deionized water:acetonitrile (90:10) |
Inj. Vol.: | 10 µL |
Mobile Phase | 10 mM ammonium acetate in water:10 mM ammonium formate in methanol with gradient program |
Flow: | 0.5 mL/min |
Detector | AB SCIEX API 4000 LC/MS/MS System |
Ion Source: | TurboIonSpray® |
Ion Mode: | ESI+ |
Mode: | MRM |
Instrument | API LC-MS/MS |
Notes | Internal Standard: 100 µL of QuEChERS internal standard mix for GC-MS analysis (cat.# 33267) and 100 µL of QuEChERS internal standard mix for LC-MS/MS analysis (cat.# 33261) were added to each 10 g sample. These internal standard mixes require no dilutions (“snap-and-shoot”) and contain compounds specified in the EN 15662 QuEChERS method. Extraction: 10 mL acetonitrile was added to a 10 gram sample of homogenized red bell pepper. After a 1 minute shake, Q-sep European method extraction salts (cat.# 26236) were added followed by another 1 minute shake. The sample was then centrifuged with a Q-sep 3000 centrifuge (cat.# 26230). dSPE: Add 1 mL extract to QuEChERS dSPE cleanup tube containing 150 mg MgSO4, 25 mg PSA, and 25 mg C18 (cat.# 26216). Shake for 30 seconds and centrifuge with Q-sep 3000 centrifuge (cat.# 26230). |
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