Sulfur Antibiotics on Raptor Biphenyl

	Peaks	t_R (min)
1.	Sulfanilamide	0.41
2.	Sulfadiazine	1.59
3.	Sulfapyridine	1.84
4.	Sulfathiazole	1.96
5.	Sulfamerazine	2.14
6.	Sulfamethazine	2.71
7.	Sulfachlorpyridazine	3.29
8.	Sulfadoxine	3.44
9.	Sulfisoxazole	3.58
10.	Sulfadimethoxine	3.98
11.	Sulfaquinoxaline	4.08

LC_FF0530

Column

Raptor Biphenyl (cat.# 9309A5E)

Dimensions:

50 mm x 3.0 mm ID

Particle Size:

2.7 µm

Temp.:

20 °C

Sample

Diluent:

0.1% Formic acid in water

Conc.:

50 µg/mL

Inj. Vol.:

5 µL

Mobile Phase

0.1% Formic acid in water

0.1% Formic acid in acetonitrile

Time (min)	Flow (mL/min)	%A	%B
0.00	1.5	95	5
2.00	1.5	90	10
4.50	1.5	65	35
4.51	1.5	95	5
5.50	1.5	95	5

Detector

Waters Acquity PDA @ 260, 4.8 nm

Instrument

Waters ACQUITY UPLC H-Class

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Sulfur Antibiotics on Raptor Biphenyl

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