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Water Soluble Vitamins by LC-MS/MS

Water Soluble Vitamins by LC-MS/MS

PeakstR (min)Conc.
(ng/mL)
Precursor IonProduct IonProduct Ion
1.Thiamine (B1)1.2650265.0122.1144.1
2.Pyridoxine (B6)1.3520170.1152.1134.1
3.Niacin (B3)1.84200123.180.178.1
4.Pantothenic acid (B5)1.94200220.090.172.1
5.Folic acid (B9)2.78500442.1295.0176.3
6.Cyanocobalamin (B12)2.92500678.3147.2359.0
7.Biotin (B7)2.93100245.0227.197.1
8.Riboflavin (B2)3.12200377.0243.1172.1
image
LC_FF0558
ColumnUltra Aqueous C18 (cat.# 9178312)
Dimensions:100 mm x 2.1 mm ID
Particle Size:3 µm
Pore Size:100 Å
Temp.:40 °C
Sample
Diluent:Water
Inj. Vol.:2 µL
Mobile Phase
A:Water + 5 mM ammonium formate + 0.1% formic acid
B:Methanol + 5 mM ammonium formate + 0.1% formic acid
Time (min)Flow (mL/min)%A%B
0.000.61000
3.500.63565
3.510.61000
6.000.61000
DetectorMS/MS
Ion Mode:ESI+
Mode:MRM
InstrumentUHPLC
NotesAutosampler temperature = 5 °C
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