Pure Chromatography
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Water Soluble Vitamins by LC-MS/MS
| Peaks | tR (min) | Conc. (ng/mL) | Precursor Ion | Product Ion | Product Ion | |
|---|---|---|---|---|---|---|
| 1. | Thiamine (B1) | 1.26 | 50 | 265.0 | 122.1 | 144.1 |
| 2. | Pyridoxine (B6) | 1.35 | 20 | 170.1 | 152.1 | 134.1 |
| 3. | Niacin (B3) | 1.84 | 200 | 123.1 | 80.1 | 78.1 |
| 4. | Pantothenic acid (B5) | 1.94 | 200 | 220.0 | 90.1 | 72.1 |
| 5. | Folic acid (B9) | 2.78 | 500 | 442.1 | 295.0 | 176.3 |
| 6. | Cyanocobalamin (B12) | 2.92 | 500 | 678.3 | 147.2 | 359.0 |
| 7. | Biotin (B7) | 2.93 | 100 | 245.0 | 227.1 | 97.1 |
| 8. | Riboflavin (B2) | 3.12 | 200 | 377.0 | 243.1 | 172.1 |
LC_FF0558
| Column | Ultra Aqueous C18 (cat.# 9178312) | ||||||||||||||||||||
| Dimensions: | 100 mm x 2.1 mm ID | ||||||||||||||||||||
| Particle Size: | 3 µm | ||||||||||||||||||||
| Pore Size: | 100 Å | ||||||||||||||||||||
| Temp.: | 40 °C | ||||||||||||||||||||
| Sample | |||||||||||||||||||||
| Diluent: | Water | ||||||||||||||||||||
| Inj. Vol.: | 2 µL | ||||||||||||||||||||
| Mobile Phase | |||||||||||||||||||||
| A: | Water + 5 mM ammonium formate + 0.1% formic acid | ||||||||||||||||||||
| B: | Methanol + 5 mM ammonium formate + 0.1% formic acid | ||||||||||||||||||||
| |||||||||||||||||||||
| Detector | MS/MS | ||||||||||||||||||||
| Ion Mode: | ESI+ | ||||||||||||||||||||
| Mode: | MRM | ||||||||||||||||||||
| Instrument | UHPLC | ||||||||||||||||||||
| Notes | Autosampler temperature = 5 °C |
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Restek Corporation, U.S., 110 Benner Circle, Bellefonte, PA 16823
Copyright © 2020 Restek Corporation. All rights reserved.
A Company of Owners
Cookies allow for a variety of features that make your visit to Restek more enjoyable. By using this website, you agree to the use of cookies in accordance with our guidelines. For more information, see our privacy policy.