Pure Chromatography
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Underivatized Amino Acids Analysis in Baby Formula on Raptor Polar X

LC_FF0579
Column | Raptor Polar X (cat.# 9311A12) | ||||||||||||||||||||||||
Dimensions: | 100 mm x 2.1 mm ID | ||||||||||||||||||||||||
Particle Size: | 2.7 µm | ||||||||||||||||||||||||
Temp.: | 30 °C | ||||||||||||||||||||||||
Sample | |||||||||||||||||||||||||
Diluent: | 20:80 Water:acetonitrile, 0.01 N HCl | ||||||||||||||||||||||||
Conc.: | Endogenous amino acids | ||||||||||||||||||||||||
Inj. Vol.: | 5 µL | ||||||||||||||||||||||||
Mobile Phase | |||||||||||||||||||||||||
A: | Water, 0.5% formic acid | ||||||||||||||||||||||||
B: | 9:1 Acetonitrile:water, 20 mM ammonium formate, pH 3.0 | ||||||||||||||||||||||||
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Detector | MS/MS | ||||||||||||||||||||||||
Ion Mode: | ESI+ | ||||||||||||||||||||||||
Mode: | MRM | ||||||||||||||||||||||||
Instrument | UHPLC | ||||||||||||||||||||||||
Notes | A 200 µL aliquot of protein hydrolysate formula (Similac ALIMENTUM) was mixed with 800 µL of acetonitrile and 10 µL of 1 N HCl. After centrifugation at 4000 rpm for 5 minutes, the supernatant was diluted 20-fold with 20:80 water:acetonitrile (0.01 N HCl) and injected for analysis. |
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