This chromatogram may contain analytes that are synonyms of your search terms.
Underivatized Amino Acids Analysis in Baby Formula on Raptor Polar X
|Column||Raptor Polar X (cat.# 9311A12)|
|Dimensions:||100 mm x 2.1 mm ID|
|Particle Size:||2.7 µm|
|Diluent:||20:80 Water:acetonitrile, 0.01 N HCl|
|Conc.:||Endogenous amino acids|
|Inj. Vol.:||5 µL|
|A:||Water, 0.5% formic acid|
|B:||9:1 Acetonitrile:200 mM ammonium formate in water (pH 3.0)|
|Notes||Sample Preparation: A 200 µL aliquot of protein hydrolysate formula (Similac ALIMENTUM) was mixed with 800 µL of acetonitrile and 10 µL of 1 N HCl. After centrifugation at 4000 rpm for 5 minutes, the supernatant was diluted 20-fold with 20:80 water:acetonitrile (0.01 N HCl) and injected for analysis.|
Mobile Phase B Preparation: To make 500 mL of mobile phase B, measure ~45 mL of water into a small beaker and add 1 mL of 10 M ammonium formate solution. Adjust pH to 3.0 by adding formic acid and then bring the volume to 50 mL with water. Combine this 50 mL ammonium formate solution (pH 3.0) with 450 mL of acetonitrile to complete the preparation.
RELATED CHROMATOGRAM LIBRARY SEARCHES
Printing tips! To print chromatograms full page, change the print settings in the print dialogue window of your PDF reader software: 1) "fit" or "fit to page", 2) "landscape" for wide chromatograms or "portrait" for tall chromatograms.