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Cannabinoids in Gummy Edibles on Raptor ARC-18

Cannabinoids in Gummy Edibles on Raptor ARC-18

PeakstR (min)mg/g*
1.Cannabidiolic acid (CBDA)2.1420.2
2.Cannabigerol (CBG)2.4050.2
3.Cannabidiol (CBD)2.5350.2
4.Cannabinol (CBN)3.7760.2
5.Δ9-Tetrahydrocannabinol (Δ9-THC)4.7530.2
6.Tetrahydrocannabinolic acid A (THCA-A)6.2790.2
*Extract from a gummy sample initially spiked at 0.2 mg/g.
image
LC_GN0667
ColumnRaptor ARC-18 (cat.# 9314A62)
Dimensions:150 mm x 2.1 mm ID
Particle Size:2.7 µm
Pore Size:90 Å
Guard Column:Raptor ARC-18 EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9314A0252)
Temp.:30 °C
SampleCannabinoids standard (cat.# 34014)
Cannabigerol (cat.# 34091)
d9-Tetrahydrocannabinol (cat.# 34067)
d9-Tetrahydrocannabinolic acid A (cat.# 34111)
Diluent:75:25 Acetonitrile:water
Conc.: Expected concentration of 4 ppm in final extract from gummy initially spiked at 0.2 mg/g.
Inj. Vol.:2 µL
Mobile Phase
A:Water, 5 mM ammonium formate, 0.1% formic acid
B:Acetonitrile, 0.1% formic acid
Time (min)Flow (mL/min)%A%B
0.000.42575
10.000.42575
DetectorUV/Vis @ 228 nm
InstrumentUHPLC
NotesGummies were manually chopped into small pieces, and 1 g of sample was weighed in a 50 mL polypropylene tube. The sample was mixed with 5 mL of water and then vigorously vortexed until all gummy pieces were fully solubilized. The sample was fortified with cannabinoids at 2 mg/g. The spiked sample was further vortexed for 30 sec. 5 mL of acetonitrile acidified with 1% acetic acid was added to the sample, and this was followed by 30 sec vortex agitation. Then, a pouch of European EN 15662 QuEChERS extraction salts (cat.# 25849) was added to the sample. The sample was vortexed for 30 sec and then centrifuged for 5 min. 100 µL of organic extract was mixed with 900 µL of 75:25 acetonitrile:water. 2 µL of final extract was injected into the HPLC-UV system.
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