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UHPLC Analysis of 21 Cannabinoids on Raptor ARC-18 1.8 μm by LC-UV

UHPLC Analysis of 21 Cannabinoids on Raptor ARC-18 1.8 μm by LC-UV

PeakstR (min)
1.Cannabidivarinic acid (CBDVA)0.813
2.Cannabidivarin (CBDV)0.888
3.Cannabidiolic acid (CBDA)1.102
4.Cannabigerolic acid (CBGA)1.172
5.Cannabigerol (CBG)1.222
6.Cannabidiol (CBD)1.278
7.Tetrahydrocannabivarin (THCV)1.398
8.Cannabichromevarin (CBCV)1.679
9.Tetrahydrocannabivarinic acid (THCVA)1.768
10.Cannabinol (CBN)1.872
PeakstR (min)
11.Cannabidiphorol (CBDP)2.088
12.Cannabinolic acid (CBNA)2.222
13.Δ9-Tetrahydrocannabinol (Δ9-THC)2.325
14.Δ8-Tetrahydrocannabinol (Δ8-THC)2.394
15.Cannabicyclol (CBL)2.740
16.Cannabichromene (CBC)2.882
17.Tetrahydrocannabinolic acid A (THCA-A)3.065
18.Cannabichromenic acid (CBCA)3.418
19.Cannabicyclolic acid (CBLA)3.620
20.Tetrahydrocannabiphorol (THCP)4.273
21.Cannabicitran (CBT)4.552
image
LC_GN0670
ColumnRaptor ARC-18 (cat.# 931421E)
Dimensions:100 mm x 3.0 mm ID
Particle Size:1.8 µm
Pore Size:90 Å
Guard Column:UltraShield UHPLC precolumn filter, 0.2 μm frit (cat.# 25809)
Temp.:30 °C
SampleCannabidivarinic acid (CBDVA) (cat.# 34134)
Cannabidivarin (CBDV) (cat.# 34123)
Cannabidiolic acid (CBDA) (cat.# 34094)
Cannabigerolic acid (CBGA) (cat.# 34135)
Cannabigerol (CBG) (cat.# 34091)
Cannabidiol (CBD) (cat.# 34011)
Tetrahydrocannabivarin (THCV) (cat.# 34100)
Cannabinol (CBN) (cat.# 34010)
d9-Tetrahydrocannabinol (d9-THC) (cat.# 34067)
d8-Tetrahydrocannabinol (d8-THC) (cat.# 34090)
Cannabicyclol (CBL) (cat.# 34130)
Cannabichromene (CBC) (cat.# 34092)
d9-Tetrahydrocannabinolic acid A (THCA-A) (cat.# 34111)
Compounds not present in these mixes were obtained separately.
Diluent:Methanol
Conc.:50 ppm
Inj. Vol.:1 µL
Mobile Phase
A:Water, 5 mM ammonium formate, 0.1% formic acid
B:Acetonitrile, 0.1% formic acid
Time (min)Flow (mL/min)%A%B
0.0012575
512575
DetectorUV/Vis @ 228 nm
InstrumentUHPLC
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