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Vitamins B1, B2, B3, and B6 on Ultra C8

Vitamins B1, B2, B3, and B6 on Ultra C8

PeakstR (min)
1.Vitamin B3 (nicotinamide)2.732
2.Vitamin B6 (pyridoxine)5.615
PeakstR (min)
3.Vitamin B2 (riboflavin)8.719
4.Vitamin B1 (thiamine)19.004
image
LC_PH0497
ColumnUltra C8 (cat.# 9103575)
Dimensions:250 mm x 4.6 mm ID
Particle Size:5 µm
Pore Size:100 Å
Temp.:50 °C
Sample
Diluent:Water:methanol (80:20)
Conc.:50 µg/mL each component
Inj. Vol.:5 µL
Mobile Phase0.008M sodium 1-hexanesulfonate:methanol:glacial acetic acid:triethylamine (817.5:175:7.5:0.2)
Flow:2.0 mL/min
DetectorUV/Vis @ 270 nm
InstrumentShimadzu UFLCXR
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