Pure Chromatography
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www.restek.com
Vitamins B1, B2, B3, and B6 on Ultra C8
| Peaks | tR (min) | |
|---|---|---|
| 1. | Vitamin B3 (nicotinamide) | 2.732 |
| 2. | Vitamin B6 (pyridoxine) | 5.615 |
| Peaks | tR (min) | |
|---|---|---|
| 3. | Vitamin B2 (riboflavin) | 8.719 |
| 4. | Vitamin B1 (thiamine) | 19.004 |
LC_PH0497
| Column | Ultra C8 (cat.# 9103575) |
| Dimensions: | 250 mm x 4.6 mm ID |
| Particle Size: | 5 µm |
| Pore Size: | 100 Å |
| Temp.: | 50 °C |
| Sample | |
| Diluent: | Water:methanol (80:20) |
| Conc.: | 50 µg/mL each component |
| Inj. Vol.: | 5 µL |
| Mobile Phase | 0.008M sodium 1-hexanesulfonate:methanol:glacial acetic acid:triethylamine (817.5:175:7.5:0.2) |
| Flow: | 2.0 mL/min |
| Detector | UV/Vis @ 270 nm |
| Instrument | Shimadzu UFLCXR |
RELATED CHROMATOGRAM LIBRARY SEARCHES
Printing tips! To print chromatograms full page, change the print settings in the print dialogue window of your PDF reader software: 1) "fit" or "fit to page", 2) "landscape" for wide chromatograms or "portrait" for tall chromatograms.
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Restek Corporation, U.S., 110 Benner Circle, Bellefonte, PA 16823
Copyright © 2019 Restek Corporation. All rights reserved.
A Company of Owners
Cookies allow for a variety of features that make your visit to Restek more enjoyable. By using this website, you agree to the use of cookies in accordance with our guidelines. For more information, see our privacy policy.