Pure Chromatography
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Vitamins B1, B2, B3, and B6 on Ultra C8
Peaks | tR (min) | |
---|---|---|
1. | Vitamin B3 (nicotinamide)![]() | 2.732 |
2. | Vitamin B6 (pyridoxine)![]() | 5.615 |
Peaks | tR (min) | |
---|---|---|
3. | Vitamin B2 (riboflavin)![]() | 8.719 |
4. | Vitamin B1 (thiamine) | 19.004 |

LC_PH0497
Column | Ultra C8 (cat.# 9103575) |
Dimensions: | 250 mm x 4.6 mm ID |
Particle Size: | 5 µm |
Pore Size: | 100 Å |
Temp.: | 50 °C |
Sample | |
Diluent: | Water:methanol (80:20) |
Conc.: | 50 µg/mL each component |
Inj. Vol.: | 5 µL |
Mobile Phase | 0.008M sodium 1-hexanesulfonate:methanol:glacial acetic acid:triethylamine (817.5:175:7.5:0.2) |
Flow: | 2.0 mL/min |
Detector | UV/Vis @ 270 nm |
Instrument | Shimadzu UFLCXR |
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