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Septum Bleed Causing Significant Interference with Target Analytes

ColumnRtx-5MS, 30 m, 0.25 mm ID, 0.25 µm (cat.# 12623)
SampleAmphetamine, methamphetamine, MDA, MDMA, and MDEA extracted from methanol and HFAA derivatized
Conc.:100 µg/mL each
Inj. Vol.:1 µL splitless (hold 0.5 min)
Liner:3.5 mm Custom splitless inlet liner w/ standard deactivated wool
Inj. Temp.:220 °C
Oven Temp.:70 °C (hold 1 min) to 290 °C at 15 °C/min (hold 4 min)
Carrier GasHe, constant flow
Flow Rate:1.25 mL/min
Transfer Line Temp.:280 °C
Ionization Mode:EI
Scan Range:43-450 amu