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Underivatized Cholesterol on Rxi-5ms (Isothermal)

ColumnRxi -5ms, 15 m, 0.25 mm ID, 0.25 µm (cat.# 13420)
Sample1,000 μg/mL cholesterol in DMF
1,000 μg/mL 5-α-cholestane in hexane
25 ng cholesterol, 150 ng 5-α-cholestane on column
Inj. Vol.:1 µL split (split ratio 20:1)
Liner:Splitless taper (4 mm) w/wool (cat.# 22405)
Inj. Temp.:250 °C
Oven Temp.:300 °C (hold 10 min)
Carrier GasHe, constant pressure (9.7 psi, 66.9 kPa)
Linear Velocity:24 cm/sec @ 200 °C
DetectorFID @ 340 °C