Welcome to the New Restek.com! Take a tour of the new site and feel free to send us your feedback.
Your web browser will no longer be supported by Restek.com as of 30 June 2021.
To avoid any interruption in access or functionality, install a current-generation web browser now. Learn more.

QuEChERS Extract of Cannabis on Rxi-17Sil MS and Rxi-5ms by GCxGC-TOFMS (Zoomed in on cannabinoids region)

GC_FF1209
ColumnRxi-17Sil MS 30 m, 0.25 mm ID, 0.25 µm (cat.# 14123)
Rxi-5ms 0.9 m, 0.18 mm ID, 0.18 µm,
Injection
Inj. Vol.:1 µL split (split ratio 20:1)
Liner:Premium 4 mm Precision Inlet Liner w/Wool (cat.# 23305.1)
Inj. Temp.:275 °C
Oven
Oven Temp.:Rxi-17Sil MS: 40 °C (hold 1 min) to 320 °C at 5 °C/min (hold 3 min)
Rxi-5ms: 45 °C (hold 1 min) to 325 °C at 5 °C/min (hold 3 min)
Carrier GasHe, corrected constant flow (2 mL/min)
Modulation
Modulator Temp. Offset:20 °C
Second Dimension Separation Time:2 sec
Hot Pulse Time:0.6 sec
Cool Time between Stages:0.4 sec
DetectorTOFMS
Transfer Line Temp.:310 °C
Analyzer Type:TOF
Source Temp.:250 °C
Electron Energy:70 eV
Mass Defect:-20 mu/100 u
Solvent Delay Time:4.5 min
Tune Type:PFTBA
Ionization Mode:EI
Acquisition Range:45-500 amu
Spectral Acquisition Rate:200 spectra/sec
InstrumentLECO Pegasus 4D GCxGC-TOFMS
NotesSample Preparation:
Sample: Up to 2 g of finely ground cannabis in a 50 mL polypropylene centrifuge tube.

Extraction: Add 10 mL acetonitrile and 10 mL of organic-free water, then shake thoroughly. Soak 1 hour, then aggressively vortex 30 minutes. Add Q-sep packet (cat.# 26235), shake 1 minute, then centrifuge 5 minutes at 3,000 g with Q-sep 3000 centrifuge (cat.# 26230).