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Nutritional Labeling FAMEs on Rt-2560 by AOCS Method Ce-1j-07

PeakstR (min)Conc.
Structural Nomenclature
1.Methyl caproate7.5240C6:0
2.Methyl octanoate7.8840C8:0
3.Methyl decanoate8.4840C10:0
4.Methyl undecanoate8.9320C11:0
5.Methyl dodecanoate9.5140C12:0
6.Methyl tridecanoate10.2720C13:0
7.Methyl myristate11.2740C14:0
8.Methyl myristoleate12.4820C14:1 (c9)
9.Methyl pentadecanoate12.5720C15:0
10.Methyl pentadecenoate14.1520C15:1 (C10)
11.Methyl palmitate14.2860C16:0
12.Methyl palmitoleate15.9820C16:1 (c9)
13.Methyl heptadecanoate16.5120C17:0
14.Methyl heptadecenoate18.6820C17:1 (c10)
15.Methyl stearate19.4340C18:0
16.Methyl octadecenoate21.0820C18:1 (t9)
17.Methyl oleate21.8540C18:1 (c9)
18.Methyl linolelaidate24.0920C18:2 (t9,t12)
PeakstR (min)Conc.
Structural Nomenclature
19.Methyl linoleate26.1420C18:2 (c9,c12)
20.Methyl arachidate28.2540C20:0
21.Methyl linolenate29.9820C18:3 (c6,c9,c12)
22.Methyl eicosenoate32.0520C20:1 (c11)
23.Methyl linolenate32.4120C18:3 (c9,c12,c15)
24.Methyl heneicosanoate33.6620C21:0
25.Methyl eicosadienoate35.3320C20:2 (c11,c14)
26.Methyl behenate36.6440C22:0
27.Methyl eicosatrienoate37.4420C20:3 (c8,c11,c14)
28.Methyl erucate38.5120C22:1 (c13)
29.Methyl eicosatrienoate38.7220C20:3 (c11,c14,c17)
30.Methyl arachidonate39.1220C20:4 (c5,c8,c11,c14)
31.Methyl tricosanoate39.7420C23:0
32.Methyl docosadienoate41.6420C22:2 (c13,c16)
33.Methyl eicosapentaenoate43.0720C20:5 (c5,c8,c11,c14,c17)
34.Methyl lignocerate43.1140C24:0
35.Methyl nervonate45.3320C24:1 (c15)
36.Methyl docosahexaenoate54.0220C22:6 (c4,c7,c10,c13,c16,c19)
ColumnRt-2560, 100 m, 0.25 mm ID, 0.20 µm (cat.# 13198)
Standard/SampleFood industry FAME mix (cat.# 35077)
Conc.:1,000 µg/mL
Inj. Vol.:1 µL split (split ratio 20:1)
Liner:Premium 4 mm Precision liner w/wool (cat.# 23305.1)
Inj. Temp.:235 °C
Oven Temp.:180 °C (hold 32 min) to 215 °C at 20 °C/min (hold 31.25 min)
Carrier GasHe, constant flow
Flow Rate:2.0 mL/min
DetectorFID @ 325 °C
Make-up Gas Flow Rate:45 mL/min
Make-up Gas Type:N2
Hydrogen flow:30 mL/min
Air flow:300 mL/min
Data Rate:20 Hz
InstrumentAgilent 7890A GC
NotesC4:0 Methyl butyrate (623-42-7) elutes in the solvent front.