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Rxi-65TG: High-Temperature Stability Overlay

GC_FF1287
Peaks
1.Tripalmitin (PPP)
2.1,2-Palmitin-3-stearin (PPS)
3.1,3-Palmitin-2-olein (POP)
4.1,2-Palmitin-3-linolein (PPL)
5.1-Palmitin-2-olein-3-stearin (POS)
6.1,2-Olein-3-palmitin (POO)
7.1-Palmitin-2-linolein-3-olein (PLO)
8.1,2-Stearin-3-olein (SSO)
9.Triolein (OOO)
10.1,2-Olein-3-linolein (OOL)
ColumnRxi-65TG, 30 m, 0.25 mm ID, 0.1 µm (cat.# 17108)
SamplePalm oil analytical standard
Mono/diacylglycerol custom mix
Diluent:Isooctane
Injection
Inj. Vol.:1 µL split (split ratio 100:1)
Liner:Topaz 4.0 mm ID Precision inlet liner w/wool (cat.# 23305)
Inj. Temp.:360 °C
Split Vent Flow Rate:200 mL/min
Oven
Carrier GasH2, constant flow
Flow Rate:2 mL/min
DetectorFID @ 360 °C
Make-up Gas Flow Rate:30 mL/min
Make-up Gas Type:N2
Hydrogen flow:40 mL/min
Air flow:370 mL/min
Data Rate:50 Hz
InstrumentAgilent 7890B GC
NotesSample prep:
~50 mg of a palm oil standard (which contains naturally occurring triglycerides) was fortified with the following mono- and diacylglycerol standards. The mixture was then brought up to 10 mL in isooctane, giving a final mono- and diglycerides concentration of ~100 μg/mL.
1. 1,2-Dipalmitin
2. 1,3-Dipalmitin
3. 1,2-Distearin
4. 1,3-Distearin
5. 1-Monopalmitin
6. 2-Monopalmitin
7. 1-Monostearin

Testing Conditions:
Six injections of fortified palm oil were made under the following conditions. Extended final hold times were used to challenge column stability. After each 24-hour challenge period, column performance was assessed using an injection with a final hold time that is typical for triglyceride analysis.

Injection 1: 100 °C (hold 1 min) to 325 °C at 30 °C/min to 345 °C at 1 °C/min to 360 °C at 5 °C/min (hold 12 hr)
Injection 2: 100 °C (hold 1 min) to 325 °C at 30 °C/min to 345 °C at 1 °C/min to 360 °C at 5 °C/min (hold 12 hr)
Injection 3: 100 °C (hold 1 min) to 325 °C at 30 °C/min to 345 °C at 1 °C/min to 360 °C at 5 °C/min (hold 5 min)
Injection 4: 100 °C (hold 1 min) to 325 °C at 30 °C/min to 345 °C at 1 °C/min to 360 °C at 5 °C/min (hold 12 hr)
Injection 5: 100 °C (hold 1 min) to 325 °C at 30 °C/min to 345 °C at 1 °C/min to 360 °C at 5 °C/min (hold 12 hr)
Injection 6: 100 °C (hold 1 min) to 325 °C at 30 °C/min to 345 °C at 1 °C/min to 360 °C at 5 °C/min (hold 5 min)