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Food Industry FAMEs on Rt-2560 by AOAC Method 996.06 Using H2

PeakstR (min)Conc. (µg/mL)Structural Nomenclature
1.Methyl butyrate6.621400C4:0
2.Methyl caproate7.711400C6:0
3.Methyl octanoate9.556400C8:0
4.Methyl decanoate12.094400C10:0
5.Methyl undecanoate13.506200C11:0
6.Methyl dodecanoate14.943400C12:0
7.Methyl tridecanoate16.372200C13:0
8.Methyl myristate17.772400C14:0
9.Methyl myristoleate18.916200C14:1 (c9)
10.Methyl pentadecanoate19.127200C15:0
11.Methyl pentadecenoate20.252200C15:1 (c10)
12.Methyl palmitate20.436600C16:0
13.Methyl palmitoleate21.361200C16:1 (c9)
14.Methyl heptadecanoate21.691200C17:0
15.Methyl heptadecenoate22.595200C17:1 (c10)
16.Methyl stearate22.899400C18:0
17.Methyl octadecenoate23.442200C18:1 (t9)
18.Methyl oleate23.677400C18:1 (c9)
PeakstR (min)Conc. (µg/mL)Structural Nomenclature
19.Methyl linolelaidate24.292200C18:2 (t9,t12)
20.Methyl linoleate24.811200C18:2 (c9,c12)
21.Methyl arachidate25.171400C20:0
22.Methyl linolenate25.637200C18:3 (c6,c9,c12)
23.Methyl eicosenoate25.903200C20:1 (c11)
24.Methyl linolenate26.081200C18:3 (c9,c12,c15)
25.Methyl heneicosanoate26.239200C21:0
26.Methyl eicosadienoate26.980200C20:2 (c11,c14)
27.Methyl behenate27.274400C22:0
28.Methyl eicosatrienoate27.763200C20:3 (c8,c11,c14)
29.Methyl erucate27.981200C22:1 (c13)
30.Methyl eicosatrienoate28.190200C20:3 (c11,c14,c17)
31.Methyl arachidonate28.286200C20:4 (c5,c8,c11,c14)
32.Methyl tricosanoate28.381200C23:0
33.Methyl docosadienoate29.071200C22:2 (c13,c16)
34.Methyl lignocerate29.341400C24:0
35.Methyl eicosapentaenoate29.737200C20:5 (c5,c8,c11,c14,c17)
36.Methyl nervonate30.131200C24:1 (C15)
37.Methyl docosahexaenoate33.132200C22:6 (c4,c7,c10,c13,c16,c19)
ColumnRt-2560, 100 m, 0.25 mm ID, 0.20 µm (cat.# 13198)
Standard/SampleFood industry FAME mix (cat.# 35077)
Conc.:10,000 µg/mL total concentration
Inj. Vol.:1 µL split (split ratio 20:1)
Liner:Topaz 4.0 mm ID Precision inlet liner w/wool (cat.# 23305)
Inj. Temp.:250 °C
Oven Temp.:100 °C (hold 1.05 min) to 240 °C at 5.3 °C/min (hold 8.5 min)
Carrier GasH2, constant flow
Flow Rate:1.75 mL/min
DetectorFID @ 250 °C
Constant Column + Constant Make-up:52 mL/min
Hydrogen flow:40 mL/min
Air flow:400 mL/min
Data Rate:50 Hz
InstrumentAgilent 7890A GC
NotesResolution of all peaks >1.5
Method translated from He using the EZGC method translator (www.restek.com/FAMEH2).