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Food Industry FAMEs on Rt-2560 by AOCS Method Ce-1h-05

PeakstR (min)Conc. (µg/mL)Structural Nomenclature
1.Methyl caproate5.830400C6:0
2.Methyl octanoate6.092400C8:0
3.Methyl decanoate6.539400C10:0
4.Methyl undecanoate6.870200C11:0
5.Methyl dodecanoate7.302400C12:0
6.Methyl tridecanoate7.866200C13:0
7.Methyl myristate8.603400C14:0
8.Methyl myristoleate9.509200C14:1 (c9)
9.Methyl pentadecanoate9.563200C15:0
10.Methyl pentadecenoate10.733200C15:1 (C10)
11.Methyl palmitate10.822600C16:0
12.Methyl palmitoleate12.080200C16:1 (c9)
13.Methyl heptadecanoate12.453200C17:0
14.Methyl heptadecenoate14.065200C17:1 (c10)
15.Methyl stearate14.597400C18:0
16.Methyl octadecenoate15.817200C18:1 (t9)
17.Methyl oleate16.393400C18:1 (c9)
18.Methyl linolelaidate18.037200C18:2 (t9,t12)
PeakstR (min)Conc. (µg/mL)Structural Nomenclature
19.Methyl linoleate19.563200C18:2 (c9,c12)
20.Methyl arachidate21.048400C20:0
21.Methyl linolenate22.417200C18:3 (c6,c9,c12)
22.Methyl eicosenoate23.849200C20:1 (c11)
23.Methyl linolenate24.242200C18:3 (c9,c12,c15)
24.Methyl heneicosanoate25.794200C21:0
25.Methyl eicosadienoate29.073200C20:2 (c11,c14)
26.Methyl behenate32.095400C22:0
27.Methyl eicosatrienoate33.613200C20:3 (c8,c11,c14)
28.Methyl erucate36.531200C22:1 (c13)
29.Methyl eicosatrienoate36.727200C20:3 (c11,c14,c17)
30.Methyl arachidonate37.548200C20:4 (c5,c8,c11,c14)
31.Methyl tricosanoate40.227200C23:0
32.Methyl docosadienoate45.045200C22:2 (c13,c16)
33.Methyl eicosapentaenoate48.043200C20:5 (c5,c8,c11,c14,c17)
34.Methyl lignocerate51.086400C24:0
35.Methyl nervonate58.002200C24:1 (c15)
36.Methyl docosahexaenoate86.473200C22:6 (c4,c7,c10,c13,c16,c19)
ColumnRt-2560, 100 m, 0.25 mm ID, 0.20 µm (cat.# 13198)
SampleFood industry FAME mix (cat.# 35077)
Conc.:10,000 µg/mL total concentration
Inj. Vol.:1 µL split (split ratio 20:1)
Liner:Topaz 4.0 mm ID Precision inlet liner w/wool (cat.# 23305)
Inj. Temp.:250 °C
Oven Temp.:180 °C (hold 90 min)
Carrier GasH2, constant flow
Flow Rate:1.75 mL/min
DetectorFID @ 250 °C
Constant Column + Constant Make-up:52 mL/min
Hydrogen flow:40 mL/min
Air flow:400 mL/min
Data Rate:50 Hz
InstrumentAgilent 7890A GC
NotesHydrogen flow optimized to achieve effective linear velocity (www.restek.com/FAMEH2).
C4:0 Methyl butyrate (623-42-7) elutes in the solvent front.