|Column||Rxi-PAH, 40 m, 0.18 mm ID, 0.07 µm (cat.# 49316)|
|Sample||Custom PAH standard|
|Inj. Vol.:||0.5 µL pulsed splitless|
|Liner:||Premium 2 mm single taper w/wool (cat.# 23316.1)|
|Inj. Temp.:||275 °C|
|Pulse Pressure:||80 psi (551.6kPa)|
|Pulse Time:||0.6 min|
|Purge Flow:||40 mL/min|
|Oven Temp.:||110 °C (hold 1 min) to 210 °C at 35 °C/min to 260 °C at 3 °C/min to 350 °C at 10 °C/min (hold 4.5 min)|
|Carrier Gas||He, constant flow|
|Flow Rate:||1.4 mL/min|
|Detector||FID @ 360 °C|
|Make-up Gas Flow Rate:||45 mL/min|
|Make-up Gas Type:||N2|
|Hydrogen flow:||40 mL/min|
|Air flow:||450 mL/min|
|Instrument||Agilent 7890A GC|
|Notes||The experiment was carried out as follows: |
- Oysters and paprika were extracted using QuEChERS methodology.
- dSPE cleanup was performed on oyster extracts.
- Cartridge cleanup was performed on paprika extracts.
- Oyster and paprika extracts were injected, interspersed with solvent standard, to monitor performance.
1 m was trimmed from the column and the liner was changed during the course of the experiment.