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Medical Cannabis Cannabinoids and Internal Standards on Rxi-35Sil MS

GC_FS0549
PeaksConc.
(µg/mL)
tR (sec)
1.Phencyclidine (IS)5073.4
2.Cannabidivarin100115.2
3.Tetrahydrocannabivarin100130.3
4.Cannabichromene100138.3
5.Cannabidiol100150.0
6.Δ8-Tetrahydrocannabinol100152.1
7.Δ9-Tetrahydrocannabinol100156.1
8.Cannabigerol100159.5
9.Cannabinol100165.6
10.Prazepam (IS)50192.4
ColumnRxi-35Sil MS, 15 m, 0.25 mm ID, 0.25 µm (cat.# 13820)
SamplePhencyclidine (cat.# 34027)
Cannabichromene (CBC) (cat.# 34092)
Cannabinoids standard (cat.# 34014)
delta-8-Tetrahydrocannabinol (THC) (cat.# 34090)
Cannabigerol (CBG) (cat.# 34091)
Prazepam (cat.# 34055)
Injection
Inj. Vol.:1 µL split (split ratio 20:1)
Liner:Premium 4 mm Precision liner w/wool (cat.# 23305.5)
Inj. Temp.:250 °C
Oven
Oven Temp.:190 °C (hold 0.1 min) to 330 °C at 35 °C/min (hold 0.9 min)
Carrier GasH2, constant flow
Flow Rate:2.5 mL/min
DetectorFID @ 350 °C
Constant Column + Constant Make-up:50 mL/min
Make-up Gas Type:N2
Hydrogen flow:40 mL/min
Air flow:450 mL/min
Data Rate:20 Hz
InstrumentAgilent/HP6890 GC
NotesCannabidivarin and tetrahydrocannabivarin standards were obtained from BOC Sciences.