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Cholesterol and 5α-Cholestane on DiatoSorb-WHP Packed Column

GC_PC1300
Column3% OV-101, 100/120 mesh on DiatoSorb-WHP, SilcoSmooth tubing, 1 m, 1/8 in OD, 2 mm ID
SampleCholesterol, 5α-cholestane
Diluent:Chloroform
Conc.:1,000 µg/mL
Injection
Inj. Vol.:1.0 µL on-column
Inj. Temp.:220 °C
Oven
Oven Temp.:250 °C (hold 8 min)
Carrier GasHe, constant flow
Flow Rate:40 mL/min
DetectorFID @ 260 °C
InstrumentAgilent 7890B GC
NotesColumn inertness results in symmetrical peak shape and almost no adsorption of active compounds.