Welcome to the New Restek.com! Take a tour of the new site and feel free to send us your feedback.
Your web browser will no longer be supported by Restek.com as of 30 June 2021.
To avoid any interruption in access or functionality, install a current-generation web browser now. Learn more.

Food Industry FAME on FAMEWAX by EN14103 (2011)

GC_PC1332
PeakstR (min)Conc.
(mg/mL)
Structural Nomenclature
1.Methyl capronate3.6291.2C6:0
2.Methyl caprylate6.2371.2C8:0
3.Methyl caprate8.7871.2C10:0
4.Methyl undecanoate9.9710.6C11:0
5.Methyl laurate11.1051.2C12:0
6.Methyl tridecanoate12.1790.6C13:0
7.Methyl myristate13.2151.2C14:0
8.Methyl myristoleate13.5490.6C14:1 (cis-9)
9.Methyl pentadecanoate14.1960.6C15:0
10.Methyl pentadecenoate14.5240.6C15:1 (cis-10)
11.Methyl palmitate15.1521.8C16:0
12.Methyl palmitoleate15.3550.6C16:1 (cis-9)
13.Methyl margarate16.0520.6C17:0
14.Methyl heptadecenoate16.2610.6C17:1 (cis-10)
15.Methyl stearate16.9951.2C18:0
16.Methyl oleate17.1561.2C18:1 (cis-9)
17.Methyl elaidate17.1681.2C18:1 (trans-9)
18.Methyl linoleate17.5830.6C18:2 (all-cis-9,12)
PeakstR (min)Conc.
(mg/mL)
Structural Nomenclature
19.Methyl linolelaidate17.6410.6C18:2 (all-trans-9,12)
20.Methyl γ-linolenate17.8740.6C18:3 (all-cis-6,9,12)
21.Methyl nonadecanoate18.0522.0C19:0
22.Methyl α-linolenate18.2230.6C18:3 (all-cis-9,12,15)
23.Methyl arachidate19.0751.2C20:0
24.Methyl (Z)-11-eicosenoate19.2550.6C20:1 (cis-11)
25.Methyl 11,14-eicosadienoate19.7610.6C20:2 (all-cis-11,14)
26.Methyl eicosa-8,11,14-trienoate20.0460.6C20:3 (all-cis-8,11,14)
27.Methyl heneicosanoate20.1970.6C21:0
28.Methyl arachidonate20.2900.6C20:4 (all-cis-5,8,11,14)
29.Methyl 11,14,17-eicosatrienoate20.4880.6C20:3 (all-cis-11,14,17)
30.Methyl 5,8,11,14,17-eicosapentanoate21.0360.6C20:5 (all-cis-5,8,11,14,17)
31.Methyl behenate21.391.2C22:0
32.Methyl erucate21.5950.6C22:1 (cis-13)
33.Methyl docosadienoate22.1500.6C22:2 (all-cis-13,16)
34.Methyl tricosanoate22.5840.6C23:0
35.Methyl lignocerate23.8261.2C24:0
36.Methyl docosahexaenoate23.8630.6C22:6 (all-cis-4,7,10,13,16,19)
37.Methyl nervonate24.0550.6C24:1 (cis-15)
ColumnFAMEWAX, 30 m, 0.25 mm ID, 0.25 µm (cat.# 12497)
SampleFood industry FAME mix (cat.# 35077)
Methyl nonadecanoate (cat.# 35055)
Diluent:Standard cat.# 35055 was dissolved in toluene.
Injection
Inj. Vol.:1 µL split (split ratio 100:1)
Liner:Topaz 4.0 mm ID Precision inlet liner w/wool (cat.# 23305)
Inj. Temp.:240 °C
Oven
Oven Temp.:60 °C (hold 2 min) to 200 °C at 10 °C/min to 240 °C at 5 °C/min (hold 7 min)
Carrier GasH2, constant flow
Flow Rate:1.7 mL/min
DetectorFID @ 250 °C
InstrumentAgilent 7890B GC
NotesThis chromatogram is an overlay of two injections: food industry FAME standard (black) and C19:0 methyl ester in toluene (red). An excellent separation of C19:0 (used in EN 14103 as an internal standard) and the most prevalent FAMEs found in biodiesel blends was achieved. Note that C4:0 from the food industry FAME standard elutes in the solvent front.