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Regulated Carcinogenic Aryl Amines Resulting From Reductive Cleavage of Azo Dyes on Rxi-35Sil MS

GC_QA0095
PeakstR (min)Conc.
(µg/mL)
Molecular Ion (m/z)
1.o-Toluidine3.1510107
2.o-Anisidine4.3610123
3.4-Chloroaniline4.7710127
4.p-Cresidine5.4610137
5.2,4,5-Trimethylaniline5.5210135
6.3-Chloro-o-toluidine5.8410141
7.4-Chloro-o-toluidine5.9610141
8.2,4-Diaminotoluene7.5810122
9.2,4,5-Trichloroaniline (IS)9.1330196
10.2-Naphthylamine9.7210143
11.2-Aminobiphenyl9.8410169
12.2-Amino-4-nitrotoluene10.4910152
PeakstR (min)Conc.
(µg/mL)
Molecular Ion (m/z)
13.4-Aminobiphenyl12.1210169
14.p-Aminoazobenzene15.9310197
15.4,4'-Oxydianiline16.6010200
16.4,4'-Diaminodiphenylmethane16.7010198
17.Benzidine16.7710184
18.o-Aminoazotoluene17.5210225
19.3,3'-Dimethyl-4,4'-diaminodiphenylmethane18.2310226
20.3,3'-Dimethylbenzidine18.4810212
21.4,4’-Thiodianiline19.4310216
22.3,3'-Dichlorobenzidine19.7910252
23.4,4'-Methylenebis(2-chloroaniline)19.7910266
24.3,3'-Dimethoxybenzidine19.9110244
Elution order of positional isomers was verified with individual standards.
ColumnRxi-35Sil MS, 30 m, 0.25 mm ID, 0.25 µm (cat.# 13823)
SampleAccuStandard carcinogenic aryl amine mix (cat.# AE-000-49-R1)
Diluent:Ethyl acetate
Conc.:10 µg/mL
Injection
Inj. Vol.:1 µL split (split ratio 10:1)
Liner:Premium 4 mm Precision liner w/wool (cat.# 23305.1)
Inj. Temp.:275 °C
Oven
Oven Temp.:100 °C to 320 °C at 9.5 °C/min (hold 5 min)
Carrier GasHe, constant flow
Flow Rate:2.0 mL/min
DetectorMS
Mode:Scan
Scan Program:
GroupStart Time
(min)
Scan Range
(amu)
Scan Rate
(scans/sec)
12.030-3005.5
Transfer Line Temp.:330 °C
Analyzer Type:Quadrupole
Source Type:Inert
Drawout Plate:3 mm ID
Source Temp.:250 °C
Quad Temp.:180 °C
Electron Energy:70 eV
Solvent Delay Time:2.0 min
Tune Type:PFTBA
Ionization Mode:EI
InstrumentAgilent 7890A GC & 5975C MSD