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High Speed GC-MS of Carcinogenic Aryl Amines Resulting From Reductive Cleavage of Azo Dyes on Rxi-35Sil MS (Scan Mode)

GC_QA0096
PeakstR (min)Conc.
(µg/mL)
Molecular Ion (m/z)
1.o-Toluidine1.1410107
2.o-Anisidine1.5610123
3.4-Chloroaniline1.7110127
4.p-Cresidine1.9510137
5.2,4,5-Trimethylaniline1.9710135
6.3-Chloro-o-toluidine2.0810141
7.4-Chloro-o-toluidine2.1310141
8.2,4-Diaminotoluene2.7010122
9.2,4,5-Trichloroaniline (IS)3.2530197
10.2-Naphthylamine3.4610143
11.2-Aminobiphenyl3.5010169
12.2-Amino-4-nitrotoluene3.7310152
PeakstR (min)Conc.
(µg/mL)
Molecular Ion (m/z)
13.4-Aminobiphenyl4.3110169
14.p-Aminoazobenzene5.6610197
15.4,4'-Oxydianiline5.9010200
16.4,4'-Diaminodiphenylmethane5.9410198
17.Benzidine5.9610184
18.o-Aminoazotoluene6.2310225
19.3,3'-Dimethyl-4,4'-diaminodiphenylmethane6.4810226
20.3,3'-Dimethylbenzidine6.5710212
21.4,4’-Thiodianiline6.9210216
22.3,3'-Dichlorobenzidine7.0510252
23.4,4'-Methylenebis(2-chloroaniline)7.0510266
24.3,3'-Dimethoxybenzidine7.0910244
Elution order of positional isomers was verified with individual standards.
ColumnRxi-35Sil MS, 15 m, 0.25 mm ID, 0.25 µm (cat.# 13820)
Standard/SampleAccuStandard carcinogenic aryl amine mix (cat.# AE-000-49-R1)
Diluent:Ethyl acetate
Conc.:10 µg/mL
Injection
Inj. Vol.:1 µL split (split ratio 10:1)
Liner:Premium 4 mm Precision liner w/wool (cat.# 23305.1)
Inj. Temp.:275 °C
Oven
Oven Temp.:100 °C to 320 °C at 27 °C/min (hold 1.75 min)
Carrier GasHe, constant flow
Flow Rate:2.0 mL/min
DetectorMS
Mode:Scan
Scan Program:
GroupStart Time
(min)
Scan Range
(amu)
Scan Rate
(scans/sec)
12.030-3005.5
Transfer Line Temp.:330 °C
Analyzer Type:Quadrupole
Source Type:Inert
Drawout Plate:3 mm ID
Source Temp.:250 °C
Quad Temp.:180 °C
Electron Energy:70 eV
Solvent Delay Time:2.0 min
Tune Type:PFTBA
Ionization Mode:EI
InstrumentAgilent 7890A GC & 5975C MSD