Welcome to the New Restek.com! Take a tour of the new site and feel free to send us your feedback.

Your web browser will no longer be supported by Restek.com as of 30 June 2021.
To avoid any interruption in access or functionality, install a current-generation web browser now. Learn more.

Water Soluble Vitamins on Ultra Aqueous C18

LC_0141
PeaksConc.
(mg/mL)
1.Thiamin (B1)250
2.Ascorbic acid (C)1,000
3.Unknownn/a
4.Nicotinic acid (B3)1,000
5.Unknownn/a
6.Pantothenic acid (B5)1,000
7.Folic acid (B9)500
8.Riboflavin (B2)250
9.Methylparaben0.2
ColumnUltra Aqueous C18 (cat.# 9178575)
Dimensions:250 mm x 4.6 mm ID
Particle Size:5 µm
Pore Size:100 Å
Temp.:27 °C
Samplewater soluble vitamins
Diluent:initial dilutions of B1 and B2 basified with ammonium hydroxide
Conc.: variable
Mobile Phase
A:25 mM potassium phosphate, pH 2.00: methanol (95:5)
B:methanol: 25 mM potassium phosphate, pH 3.5 (60:40)
Time (min)%B
0-60
6.0125
11100
16100
Flow:1 mL/min
DetectorUV/Vis @ 254 nm