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Aromatic Amino Acids on Ultra Aqueous C18

LC_0146
PeaksConc.
(mg/mL)
1.Tyrosine0.2
2.Phenylalanine1.6
3.Tryptophan0.4
ColumnUltra Aqueous C18 (cat.# 9178565)
Dimensions:150 mm x 4.6 mm ID
Particle Size:5 µm
Pore Size:100 Å
Temp.:30 °C
Sample
Diluent:mobile phase A
Inj. Vol.:20 µL
Mobile Phase
A:50 mM potassium phosphate, pH 2.5
B:acetonitrile
Time (min)%B
0–55–20
5–620–5
6–13Hold at 5
Flow:1.0 mL/min
DetectorUV/Vis @ 254 nm