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Aromatic Amino Acids on Ultra Aqueous C18 (Fast Analysis)

LC_0307
PeakstR (min)Conc.
(mg/mL)
1.Tyrosine1.2861.25
2.Phenylalanine2.1970.55
3.Tryptophan2.4750.035
ColumnUltra Aqueous C18 (cat.# 9178535)
Dimensions:30 mm x 4.6 mm ID
Particle Size:5 µm
Pore Size:100 Å
Temp.:50 °C
Sample
Diluent:50mM potassium phosphate, pH 2.5
Inj. Vol.:10 µL
Mobile Phase
A:50mM potassium phosphate, pH 2.5
B:acetonitrile
Time (min)%A%B
0.001000
1.06040
1.21000
5.01000
Flow:1.0 mL/min
DetectorUV/Vis @ 254 nm