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Nitrofuran Metabolites by LC-MS/MS on Ultra C18 Column

LC_0323
PeakstR (min)Prec. IonProd. IonCollision E (V)Tube Lens
1.d5-AMOZ3.7--------
2.AMOZ3.833529110100
3.AHD5.4624913412110
4.d4-AOZ 5.49--------
5.AOZ5.5123613412120
6.SC5.62091661280
AMOZ = 3-amino-5-morpholinomethyl-2-oxazolidinone; AHD = 1-aminohydantoin hydrochloride; AOZ = 3-amino-2-oxazolidinone; SC = semicarbazide
ColumnUltra C18 (cat.# 9174312)
Dimensions:100 mm x 2.1 mm ID
Particle Size:3 µm
Pore Size:100 Å
Temp.:30 °C
Sample
Conc.:0.3 ppb each analyte
Mobile Phase
A:0.05% formic acid in methanol
B:0.05% formic acid – 5 mM NH4 formate in water
Time (min)%B
090
2.590
510
1010
1290
1590
Flow:0.2 mL/min
DetectorMS/MS triple quadrupole (Thermo Scientific Discovery)
Ion Source:Electrospray
Ion Mode:ESI+
NotesAnalyzer Parameters:
Only segment: 15 min
Data type: centroid
Scan mode: SRM product
Scan width (m/z): 0.7
Scan time (s): 0.25
Peak width: Q1: within 0.7; Q2: 0.7
Collision gas pressure (mTorr): 1.5 (argon)
Divert valve: active, with 3 positions
Positions-1° 2 min, 2° 8 min, 3° 5 min