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Protein Mix on Viva C18

LC_0324
PeakstR (min)
1.Ribonuclease A11.31
2.Cytochrome c14.65
3.Holo-transferrin16.32
4.Apomyoglobin20.34
ColumnViva C18 (cat.# 9514562)
Dimensions:150 mm x 2.1 mm ID
Particle Size:5 µm
Pore Size:300 Å
Temp.:25 °C (or ambient)
Sample
Diluent:0.10% TFA in water:0.10% TFA in acetonitrile (80:20)
Conc.:0.08 mg/mL each protein
Inj. Vol.:20 µL
Mobile Phase
A:0.10% TFA in water
B:0.10% TFA in acetonitrile
Time (min)%B
0.0020
3070
Flow:0.20 mL/min
DetectorUV/Vis @ 214 nm
NotesSample temp.: 25 °C