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Drugs of Abuse on Ultra Biphenyl

LC_CF0534
PeakstR (min)Positive MRM1Positive MRM2Positive MRM3Negative MRM1Negative MRM2Negative MRM3
1.Ecgonine0.58186.1/168.2186.1/150.9186.1/82.2------
2.Ecgonine methyl ester0.62200.2/82.2200.2/68.2200.2/65.1------
3.Morphine1.35286.1/201.3286.1/229.9286.1/181.1------
4.Amphetamine1.63136.0/91.0136.0/119.1136.0/65.0------
5.Codeine1.76300.2/165.2300.2/152.2300.2/115.2------
6.Methamphetamine1.80150.0/91.2150.0/119.0150.0/65.0------
7.6-MAM1.88328.1/165.1328.1/211.1328.1/152.2------
8.MDMA1.94194.1/163.1194.1/105.1194.1/133.0------
9.Benzoylecgonine2.14290.1/168.1290.1/105.1290.1/77.1------
10.Cocaine2.59304.2/182.1304.2/82.1304.2/105.0------
11.Cocaethylene2.84318.2/196.2318.2/82.2318.2/77.1------
12.PCP3.02244.2/86.1244.2/159.2244.2/91.1------
13.THC-COOH4.27------343.0/299.2343.0/190.8343.0/245.4
14.THC4.63315.3/193.3315.3/93.0315.3/69.1------
The instrument was operated in multi-period mode for this experiment. Period 1 was ESI+, lasting from 0.0 min to 3.5 min, Period 2 was ESI-, lasting from 3.5 min to 4.5 min, Period 3 was ESI+, lasting from 4.5 min to 6.5 min
ColumnUltra Biphenyl (cat.# 9109552)
Dimensions:50 mm x 2.1 mm ID
Particle Size:5 µm
Pore Size:100 Å
Temp.:30 °C
Sample
Diluent:0.1 Formic acid in water:0.1% formic acid in acetonitrile (98:2)
Conc.:50 ng/mL
Inj. Vol.:10 µL
Mobile Phase
A:0.1% Formic acid in water
B:0.1% Formic acid in acetonitrile
Time (min)Flow (mL/min)%A%B
0.000.6982
2.70.66040
4.50.60100
5.00.60100
5.10.6982
DetectorApplied Biosystems/MDS Sciex LC-MS/MS
Model #:API 4000
Ion Source:TurboIonSpray®
Ion Spray Voltage:+/- 4.5 kV
Curtain Gas:40 psi (275.8 kPa)
Gas 1:60 psi (413.7 kPa)
Gas 2:60 psi (413.7 kPa)
Interface Temp.:600 °C
Mode:MRM
InstrumentApplied Biosystems/MDS Sciex LC-MS/MS System