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Steroids on Raptor Biphenyl

LC_CF0559
PeakstR (min)Conc.
(µg/mL)
1.Cortisol0.7425
2.Corticosterone1.3125
3.Estradiol1.49100
4.Boldenone1.6625
5.Trenbolone1.8425
PeakstR (min)Conc.
(µg/mL)
6.Testosterone2.0525
7.Methyltestosterone2.2925
8.17-OH-progesterone2.4625
9.Androstenedione3.0225
10.Progesterone4.4825
11.25-Hydroxyvitamin D34.6925
ColumnRaptor Biphenyl (cat.# 9309A12)
Dimensions:100 mm x 2.1 mm ID
Particle Size:2.7 µm
Temp.:30 °C
Sample
Diluent:Water:acetonitrile (60:40)
Conc.:25-100 µg/mL
Inj. Vol.:1 µL
Mobile Phase
A:Water
B:Acetonitrile
Time (min)Flow (mL/min)%A%B
0.000.56040
2.500.55545
5.000.50100
5.010.56040
7.000.56040
DetectorWaters Acquity® PDA @ 254 nm
InstrumentWaters ACQUITY UPLC H-Class