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Catecholamines, Serotonin, and Related Compounds on Raptor Biphenyl

LC_CF0560
PeakstR (min)
1.Norepinephrine0.85
2.Epinephrine1.11
3.L-Dihydroxyphenylalanine1.21
4.Dopamine1.40
5.Tyrosine1.59
6.3-Methoxytyramine*2.33
7.Serotonin2.38
8.(3,4-Dihydroxyphenyl)acetic acid*2.79
9.5-Hydroxyindoleacetic acid**3.16
10.Homovanillic acid*3.22
* Dopamine metabolite, ** serotonin metabolite
ColumnRaptor Biphenyl (cat.# 9309A62)
Dimensions:150 mm x 2.1 mm ID
Particle Size:2.7 µm
Temp.:30 °C
Sample
Diluent:Water
Conc.:50 µg/mL
Inj. Vol.:1 µL
Mobile Phase
A:0.1% Formic acid in water
B:0.1% Formic acid in acetonitrile
Time (min)Flow (mL/min)%A%B
0.000.41000
4.000.43070
4.010.41000
5.000.41000
DetectorWater Acquity® PDA @ 280 nm
InstrumentWaters ACQUITY UPLC H-Class