Catecholamines, Serotonin, and Related Compounds on Raptor Biphenyl

	Peaks	t_R (min)
1.	Norepinephrine	0.85
2.	Epinephrine	1.11
3.	L-Dihydroxyphenylalanine	1.21
4.	Dopamine	1.40
5.	Tyrosine	1.59
6.	3-Methoxytyramine*	2.33
7.	Serotonin	2.38
8.	(3,4-Dihydroxyphenyl)acetic acid*	2.79
9.	5-Hydroxyindoleacetic acid**	3.16
10.	Homovanillic acid*	3.22

* Dopamine metabolite, ** serotonin metabolite

Column

Raptor Biphenyl (cat.# 9309A62)

Dimensions:

150 mm x 2.1 mm ID

Particle Size:

2.7 µm

Temp.:

30 °C

Standard/Sample

Diluent:

Water

Conc.:

50 µg/mL

Inj. Vol.:

1 µL

Mobile Phase

0.1% Formic acid in water

0.1% Formic acid in acetonitrile

Time (min)	Flow (mL/min)	%A	%B
0.00	0.4	100	0
4.00	0.4	30	70
4.01	0.4	100	0
5.00	0.4	100	0

Detector	Water Acquity^® PDA @ 280 nm
Instrument	Waters ACQUITY UPLC H-Class

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