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Benzodiazepines in Urine on Raptor Biphenyl by LC-MS/MS

LC_CF0581
PeakstR (min)Q1Q3 QuantifierQ3 Qualifier
1.7-Aminoclonazepam0.86286.0222.2250.3
2.7-Aminoflunitrazepam1.15284.2135.2240.2
3.Flurazepam1.29388.1315.1288.2
4.Midazolam1.41326.1291.2244.2
5.N-Desmethylflunitrazepam1.76300.0254.1225.2
6.Lorazepam1.79321.0275.1302.9
7.Oxazepam1.86287.1241.0104.1
8.Clonazepam1.88315.9270.2214.1
9.Nitrazepam1.88282.1236.1207.2
10.Desalkylflurazepam1.93289.0140.1165.1
PeakstR (min)Q1Q3 QuantifierQ3 Qualifier
11.2-Hydroxyethylflurazepam2.00333.1246.1166.0
12.Nordiazepam2.07271.0140.0208.0
13.Clobazam2.13301.1259.1224.0
14.Estazolam2.18294.9267.1241.0
15.Triazolam2.19343.1308.2315.0
16.Flunitrazepam2.19314.4239.1211.0
17.Temazepam2.23301.2255.0177.1
18.Alprazolam2.27309.4281.1205.0
19.Diazepam-d5 (IS)2.45290.0154.1-
20.Diazepam2.47284.8193.1222.2
ColumnRaptor Biphenyl (cat.# 9309A5E)
Dimensions:50 mm x 3.0 mm ID
Particle Size:2.7 µm
Guard Column:Raptor Biphenyl EXP 5 mm, 3.0 mm ID, 2.7 µm (cat.# 9309A0253)
Temp.:40 °C
SampleBenzodiazepine custom mix
Conc.: A 50 ng/mL standard was prepared in urine. 50 µL of 50 ng/mL urine standard was diluted with 950 µL of water prior to analysis (20x dilution).
Inj. Vol.:20 µL
Mobile Phase
A:2 mM Ammonium formate and 0.2% formic acid in water
B:0.2% Formic acid in methanol
Time (min)Flow (mL/min)%A%B
0.000.85050
2.500.8595
3.000.8595
3.010.85050
4.500.85050
DetectorABSCIEX API 4000 MS/MS
Ion Source:TurboIonSpray®
Ion Mode:ESI+
Mode:Scheduled MRM
InstrumentAPI LC-MS/MS