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Vitamin D Metabolites on Raptor ARC-18 by LC-MS/MS

LC_CF0586
PeakstR (min)Conc.
(ng/mL)
Q1Q3
1.1,25-Dihydroxyvitamin D30.88200399.4381.5
2.25-Hydroxyvitamin D31.33200401.5383.5
3.25-Hydroxyvitamin D21.41200413.5395.5
4.Vitamin D23.47200397.5379.6
5.Vitamin D33.53200385.5367.5
ColumnRaptor ARC-18 (cat.# 9314A12)
Dimensions:100 mm x 2.1 mm ID
Particle Size:2.7 µm
Temp.:40 °C
Sample
Diluent:Methanol
Conc.:200 ng/mL
Inj. Vol.:5 µL
Mobile Phase
A:0.1% Formic acid + 5 mM ammonium formate in water
B:0.1% Formic acid + 5 mM ammonium formate in methanol
Time (min)Flow (mL/min)%A%B
0.000.51090
4.000.50100
4.010.51090
6.000.51090
DetectorABSciex API 4000
Ion Source:TurboIonSpray®
Ion Mode:ESI+
InstrumentShimadzu UFLCXR