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Thyroid Hormones on Raptor Biphenyl by LC-MS/MS

LC_CF0590
PeakstR (min)Precursor IonProduct Ion
1.3,3',5-Triiodo-L-thyronine (T3)1.05651.84605.80
2.3,3',5'-Triiodo-L-thyronine (rT3)1.28651.84605.80
3.L-Thyroxine (T4)1.48777.71731.72
ColumnRaptor Biphenyl (cat.# 9309512)
Dimensions:100 mm x 2.1 mm ID
Particle Size:5 µm
Temp.:40 °C
Sample
Diluent:Water:methanol (35:65)
Conc.:1 ng/mL
Inj. Vol.:2 µL
Mobile Phase
A:0.1% Formic acid in water
B:0.1% Formic acid in methanol
Time (min)Flow (mL/min)%A%B
0.000.43565
2.000.42080
2.010.43565
3.500.43565
DetectorMS/MS
Ion Mode:ESI+
InstrumentHPLC