Welcome to the New Restek.com! Take a tour of the new site and feel free to send us your feedback.
Your web browser will no longer be supported by Restek.com as of 30 June 2021.
To avoid any interruption in access or functionality, install a current-generation web browser now. Learn more.

Coenzyme Q10 in Serum on Raptor Biphenyl by LC-MS/MS

LC_CF0611
PeakstR (min)Precursor IonProduct Ion
1.Ubiquinol-100.98882.82197.18
2.Ubiquinone-10-D91.92872.89206.16
3.Ubiquinone-101.95863.86197.12
ColumnRaptor Biphenyl (cat.# 9309552)
Dimensions:50 mm x 2.1 mm ID
Particle Size:5 µm
Pore Size:90 Å
Guard Column:Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 5 µm (cat.# 930950252)
Temp.:40 °C
Sample
Diluent:See sample preparation
Conc.: Endogenous levels in serum
Inj. Vol.:5 µL
Mobile Phase
A:0.1% formic acid, 5 mM ammonium formate in 50:50 water:acetonitrile
B:0.1% formic acid, 5 mM ammonium formate in methanol
Time (min)Flow (mL/min)%A%B
0.000.6595
2.500.60100
2.510.6595
4.000.6595
DetectorMS/MS
Ion Mode:ESI+
Mode:MRM
InstrumentUHPLC
NotesSample Preparation Procedure:
A 150 μL aliquot of serum was mixed with 2.5 μL of ubiquinone-10-D9 (10 μg/mL in methanol) and 850 μL of 1-propanol. Stirred to mix for 2 minutes and then centrifuged at 4,000 rpm for 5 minutes. Transferred a portion of extract to HPLC vial for analysis.